Immune responses to a GnRH-based anti-fertility immunogen, induced by different adjuvants and subsequent effect on vaccine efficacy

被引：25

作者：

Ferro, VA

Costa, R

Carter, KC

Harvey, MJA

Waterston, MM

Mullen, AB

Matschke, C

Mann, JFS

Colston, A

Stimson, WH

机构：

[1] Univ Strathclyde, Dept Immunol, Glasgow G4 0NR, Lanark, Scotland

[2] Univ Glasgow, Dept Small Anim Clin Studies, Sch Vet, Glasgow G61 1QH, Lanark, Scotland

[3] Univ Strathclyde, Dept Pharmaceut Sci, Glasgow G4 0NR, Lanark, Scotland

[4] Altana Pharma AG, D-78467 Constance, Germany

[5] Triveritas Ltd, Cumbria, England

来源：

VACCINE | 2004年 / 22卷 / 08期

关键词：

GnRH; anti-fertility; vaccine;

D O I：

10.1016/j.vaccine.2003.08.043

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A modified GnRH peptide (CHWSYGLRPG-NH2) was conjugated to tetanus toxoid and formulated with different adjuvants (non-ionic surfactant vesicles, aluminium hydroxide, Quil A, PLGA (poly(lactide-co-glycolide)/triacetin), and Quil A/PLGA). A comparison of the anti-fertility efficacy of the formulations was made by examining specific antibody levels, antibody subclasses, endocrine ablation and gonadal atrophy. The production of IgG2b antibody provided the best correlation for castration. PLGA was considered the most effective adjuvant as it produced a consistent anti-fertility response in all the treated animals. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：1024 / 1031

页数：8

共 28 条

[1]

Brewer JM, 1998, J IMMUNOL, V161, P4000

[2]

Conacher M, 2000, DRUG TARG D, V11, P185

[3]

Fernando GJP, 1998, SCAND J IMMUNOL, V47, P459

[4]

Ferro V A, 1996, Drug Des Discov, V14, P179