Prion protein is a component of the multimolecular signaling complex involved in T cell activation

被引:95
作者
Mattei, V
Garofalo, T
Misasi, R
Circella, A
Manganelli, V
Lucania, G
Pavan, A
Sorice, M
机构
[1] Univ Roma La Sapienza, Dipartimento Med Sperimentale & Patol, I-00161 Rome, Italy
[2] Univ Aquila, Dipartimento Med Sperimentale, I-67100 Laquila, Italy
来源
FEBS LETTERS | 2004年 / 560卷 / 1-3期
关键词
prion protein; lipid domain; raft; ganglioside; T cell activation;
D O I
10.1016/S0014-5793(04)00029-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study we analyzed the interaction of prion protein PrPC with components of glycosphingolipid-enriched microdomains in lymphoblastoid T cells. PrPC was distributed in small clusters on the plasma membrane, as revealed by immunoelectron microscopy. PrPC is present in microdomains, since it coimmunoprecipitates with GM3 and the raft marker GM1. A strict association between PrPC and Fyn was revealed by scanning confocal microscopy and coimmunoprecipitation experiments. The phosphorylation protein ZAP-70 was immuno-precipitated by anti-PrP after T cell activation. These results demonstrate that PrPC interacts with ZAP-70, suggesting that PrPC is a component of the multimolecular signaling complex within microdomains involved in T cell activation. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:14 / 18
页数:5
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