Photoactivatable fluorescent proteins

被引:356
作者
Lukyanov, KA
Chudakov, DM
Lukyanov, S
Verkhusha, VV
机构
[1] Russian Acad Sci, Inst Bioorgan Chem, Moscow 117997, Russia
[2] Univ Colorado, Dept Pharmacol, Aurora, CO 80045 USA
[3] Hlth Sci Ctr, Aurora, CO 80045 USA
关键词
D O I
10.1038/nrm1741
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The fluorescence characteristics of photoactivatable proteins can be controlled by irradiating them with light of a specific wavelength, intensity and duration. This provides unique possibilities for the optical labelling and tracking of living cells, organelles and intracellular molecules in a spatio-temporal manner. Here, we discuss the properties of the available photoactivatable fluorescent proteins and their potential applications.
引用
收藏
页码:885 / 891
页数:7
相关论文
共 55 条
  • [1] An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein
    Ando, R
    Hama, H
    Yamamoto-Hino, M
    Mizuno, H
    Miyawaki, A
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) : 12651 - 12656
  • [2] Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting
    Ando, R
    Mizuno, H
    Miyawaki, A
    [J]. SCIENCE, 2004, 306 (5700) : 1370 - 1373
  • [3] Frequent fusion and fission of plant mitochondria with unequal nucleoid distribution
    Arimura, S
    Yamamoto, J
    Aida, GP
    Nakazono, M
    Tsutsumi, N
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (20) : 7805 - 7808
  • [4] Light-driven decarboxylation of wild-type green fluorescent protein
    Bell, AF
    Stoner-Ma, D
    Wachter, RM
    Tonge, PJ
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (23) : 6919 - 6926
  • [5] Seeing the wood through the trees: A review of techniques for distinguishing green fluorescent protein from endogenous autofluorescence
    Billinton, N
    Knight, AW
    [J]. ANALYTICAL BIOCHEMISTRY, 2001, 291 (02) : 175 - 197
  • [6] Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein
    Brejc, K
    Sixma, TK
    Kitts, PA
    Kain, SR
    Tsien, RY
    Ormo, M
    Remington, SJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) : 2306 - 2311
  • [7] A monomeric red fluorescent protein
    Campbell, RE
    Tour, O
    Palmer, AE
    Steinbach, PA
    Baird, GS
    Zacharias, DA
    Tsien, RY
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (12) : 7877 - 7882
  • [8] Ultra-fast excited state dynamics in green fluorescent protein: Multiple states and proton transfer
    Chattoraj, M
    King, BA
    Bublitz, GU
    Boxer, SG
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (16) : 8362 - 8367
  • [9] Kindling fluorescent proteins for precise in vivo photolabeling
    Chudakov, DM
    Belousov, VV
    Zaraisky, AG
    Novoselov, VV
    Staroverov, DB
    Zorov, DB
    Lukyanov, S
    Lukyanov, KA
    [J]. NATURE BIOTECHNOLOGY, 2003, 21 (02) : 191 - 194
  • [10] Photoswitchable cyan fluorescent protein for protein tracking
    Chudakov, DM
    Verkhusha, VV
    Staroverov, DB
    Souslova, EA
    Lukyanov, S
    Lukyanov, KA
    [J]. NATURE BIOTECHNOLOGY, 2004, 22 (11) : 1435 - 1439