Micropipet-assisted formation of microscopic networks of unilamellar lipid bilayer nanotubes and containers

被引:87
作者
Karlsson, M
Sott, K
Cans, AS
Karlsson, A
Karlsson, R
Orwar, O [1 ]
机构
[1] Chalmers Univ Technol, Dept Phys Chem, SE-41296 Gothenburg, Sweden
[2] Chalmers Univ Technol, Microtechnol Ctr, SE-41296 Gothenburg, Sweden
[3] Univ Gothenburg, Dept Chem, SE-41296 Gothenburg, Sweden
关键词
D O I
10.1021/la0108611
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We describe a novel micropipet-assisted technique for the construction of complex, surface-immobilized two-dimensional microscopic networks of unilamellar phospholipid bilayer vesicles (1-50 pm in diameter, 10(-15)-10(-12) L) interconnected by lipid nanotubes (100-300 nm in diameter). As starting material for the construction of networks, we used twinned vesicle pairs, one of which is multilamellar and functions as a membrane donor and the other unilamellar and functions as a membrane acceptor upon manipulation. By electromechanical insertion of a pipet tip into the unilamellar vesicle followed by lateral pulling of the micropipet away from the vesicle, a nanotube was formed. Buffer solution contained in the pipet was then injected into the nanotube orifice, forming a vesicle of controlled size that was immobilized on the surface. The networks have controlled connectivity and are well-defined with regard to the container size, angle between nanotube extensions, and nanotube length. The internal fluid composition of individual vesicles is defined during the formation of a network by selection of the solution contained in the micropipet.
引用
收藏
页码:6754 / 6758
页数:5
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