Amyloid beta protein and its 3-kDa fragment are present in the axoplasm fraction of the white matter in human brain

被引:8
作者
Tokuda, T [1 ]
Kametani, F [1 ]
Tanaka, K [1 ]
Sahara, N [1 ]
Ikeda, S [1 ]
Yanagisawa, N [1 ]
机构
[1] TOKYO INST PSYCHIAT,DEPT BIOL MOLEC,TOKYO 156,JAPAN
关键词
D O I
10.1006/bbrc.1996.0863
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Production of the soluble amyloid beta-protein (A beta) precedes abnormal accumulation of A beta amyloid in the brains of subjects with Alzheimer's disease. To determine the cellular source and generating mechanisms of soluble A beta in the human brain, we separated an axoplasm fraction from the cerebral white matter and analyzed it. The axoplasm fraction contained secretory isoforms of beta-amyloid precursor protein (APP) and 11.5 kDa A beta-bearing carboxyl-terminal fragments (CTFs) of APP. Furthermore, soluble 4 kDa A beta and 3 kDa fragments of A beta (p3) were obtained from the axoplasm fraction. These results suggest that amyloidogenic 4 kDa A beta is intracellularly produced in cerebral neurons and carried through the axons in human brain. (C) 1996 Academic Press, Inc.
引用
收藏
页码:165 / 169
页数:5
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