Amyloid beta protein and its 3-kDa fragment are present in the axoplasm fraction of the white matter in human brain

被引：8

作者：

Tokuda, T ^{[1
]}

Kametani, F ^{[1
]}

Tanaka, K ^{[1
]}

Sahara, N ^{[1
]}

Ikeda, S ^{[1
]}

Yanagisawa, N ^{[1
]}

机构：

[1] TOKYO INST PSYCHIAT,DEPT BIOL MOLEC,TOKYO 156,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 223卷 / 01期

关键词：

D O I：

10.1006/bbrc.1996.0863

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Production of the soluble amyloid beta-protein (A beta) precedes abnormal accumulation of A beta amyloid in the brains of subjects with Alzheimer's disease. To determine the cellular source and generating mechanisms of soluble A beta in the human brain, we separated an axoplasm fraction from the cerebral white matter and analyzed it. The axoplasm fraction contained secretory isoforms of beta-amyloid precursor protein (APP) and 11.5 kDa A beta-bearing carboxyl-terminal fragments (CTFs) of APP. Furthermore, soluble 4 kDa A beta and 3 kDa fragments of A beta (p3) were obtained from the axoplasm fraction. These results suggest that amyloidogenic 4 kDa A beta is intracellularly produced in cerebral neurons and carried through the axons in human brain. (C) 1996 Academic Press, Inc.

引用

页码：165 / 169

页数：5

共 30 条

[11] THE IMMUNOHISTOCHEMICAL DEMONSTRATION OF SUBSEQUENCES OF THE PRECURSOR OF THE AMYLOID A4-PROTEIN IN SENILE PLAQUES IN ALZHEIMERS-DISEASE [J].