Fibronectin fragment CH-296 inhibits apoptosis and enhances ex vivo gene transfer by murine retrovirus and human lentivirus vectors independent of viral tropism in nonhuman primate CD34+ cells

被引：33

作者：

Donahue, RE

Sorrentino, BP

Hawley, RG

An, DS

Chen, ISY

Wersto, RP

机构：

[1] NIA, Ctr Gerontol Res, Flow Cytometry Unit, NIH, Baltimore, MD 21224 USA

[2] Univ Calif Los Angeles, Sch Med, AIDS Inst, Los Angeles, CA 90095 USA

[3] Amer Red Cross, Jerome H Holland Lab, Rockville, MD 20855 USA

[4] St Jude Childrens Res Hosp, Memphis, TN 38105 USA

[5] NHLBI, Hematol Branch, NIH, Bethesda, MD 21892 USA

来源：

MOLECULAR THERAPY | 2001年 / 3卷 / 03期

基金：

美国国家卫生研究院;

关键词：

CH-296; gene therapy; hematopoietic stem cells; retrovirus; lentivirus; cell cycle;

D O I：

10.1006/mthe.2001.0269

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The fibronectin fragment CH-296 improved gene transfer to cytokine-mobilized nonhuman primate CD34(+) cells irrespective of tropism to the MoMLV, GaLV, and VSV-C envelope proteins using murine stem cell virus (MSCV) and human immunodeficiency virus-1 (HIV-1)-based retrovirus vectors. For the HIV-1 lentivirus vector, CH-296 enhanced gene transfer in the absence of added hematopoietic growth factors necessary for ex vivo stem cell expansion. In the presence of CH-296, apoptosis of CD34+ cells was inhibited, and in mobilized peripheral blood CD34+ cells, cell division was stimulated as measured by cell history/tracking experiments.

引用

页码：359 / 367

页数：9

共 57 条

[1] High-efficiency transduction of human lymphoid progenitor cells and expression in differentiated T cells [J].