Characterization of genes for two-component phosphorelay mediators with a single HPt domain in Arabidopsis thaliana

被引：97

作者：

Miyata, S

Urao, T

Yamaguchi-Shinozaki, K

Shinozaki, K

机构：

[1] Minist Agr Forestry & Fisheries, JIRCAS, Biol Resources Div, Ibaraki 3058686, Japan

[2] Inst Phys & Chem Res, Tsukuba Life Sci Ctr, Plant Mol Biol Lab, Ibaraki 3050074, Japan

来源：

FEBS LETTERS | 1998年 / 437卷 / 1-2期

关键词：

two-component system; HPt domain; tissue-specific expression; Arabidopsis thaliana;

D O I：

10.1016/S0014-5793(98)01188-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three cDNAs that encode two-component phosphorelay-mediator-like proteins were cloned from Arabidopsis thaliana, Putative proteins (ATHP1-3) contain an HPt (Histidine-containing Phospho transfer)-like domain with a conserved histidine and some invariant residues that are involved in phosphorelay. Growth retardation of YPD1-disrupted yeast cells was reversed with ATHPs, which indicates that ATHPs function as phosphorelay mediators in yeast cells, The ATHP genes are expressed more in roots than in other tissues, similar to the expression of genes for a sensor histidine kinase, ATHK1, and response regulators ATRR1-4. These results suggest that ATHPs function as two-component phosphorelay mediators between sensor histidine kinase and response regulators in Arabidopsis. (C) 1998 Federation of European Biochemical Societies.

引用

页码：11 / 14

页数：4

共 33 条

[1] Hyphal development in Neurospora crassa: Involvement of a two-component histidine kinase [J].