Nanosized hybrid particles with double luminescence for biological labeling

被引:182
作者
Louis, C
Bazzi, R
Marquette, CA
Bridot, JL
Roux, S [1 ]
Ledoux, G
Mercier, B
Blum, L
Perriat, P
Tillement, O
机构
[1] Univ Lyon 1, Lab Physicochim Mat Luminescents, UMR 5620, CNRS, F-69622 Villeurbanne, France
[2] Univ Lyon 1, Lab Genie Enzymat & Biomol, UMR 5013, CNRS, F-69622 Villeurbanne, France
[3] Inst Natl Sci Appl, UMR 5510, Etud Met Phys & Phys Mat Grp, F-69621 Villeurbanne, France
关键词
D O I
10.1021/cm0480162
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Doubly luminescent core/shell structure nanoparticles were synthesized for biological detection. In the first step gadolinium oxide (Gd2O3) core doped with the luminescent Tb3+ ions was obtained by applying, with modifications, the polyol route, which allows direct precipitation of oxide nanoparticles in a polyalcohol medium. The presence of Tb3+ ions in the Gd2O3 crystalline matrix confers attractive optical properties for long-term studies and multilabeling such as a high photostability and narrow emission bands. The water sensitivity of these particles, which is detrimental for the Tb ion's luminescence, was overcome by embedding the oxide core in a functionalized polysiloxane shell prepared by hydrolysis condensation of a mixture of APTES and TEOS. This protective layer allows the dispersion of the particles in aqueous solution without loss of luminescence intensity. Moreover, the luminescence of polysiloxane-coated Gd2O3 nanoparticles is more intense than that in the case of the naked Gd2O3 core. Due to the presence of amino groups, organic dyes and biotargeting groups (nucleic acid, biotin, streptavidin) were covalently linked to the polysiloxane network. These particles are efficient for detection of biomolecules whose presence is revealed by the high fluorescence of organic dyes and/or the photostable Tb3+ ion's luminescence.
引用
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页码:1673 / 1682
页数:10
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