Mammalian mitochondria contain a soluble acyl carrier protein

被引：55

作者：

Cronan, JE

Fearnley, IM

Walker, JE

机构：

[1] MRC, Dunn Human Nutr Unit, Cambridge CB2 2XY, England

[2] Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA

来源：

FEBS LETTERS | 2005年 / 579卷 / 21期

关键词：

acyl carrier protein; mitochondrial matrix; type II fatty acid synthesis; NADH : ubiquinone oxidoreductase; complex I;

D O I：

10.1016/j.febslet.2005.07.077

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant and fungal mitochondria contain type II fatty acid synthesis systems closely related to those of bacteria in which the individual reactions are catalyzed by separate soluble proteins acting on intermediates bound to acyl carrier protein (ACP). Mammalian mitochondria are thought to synthesize fatty acids, but evidence for the key ACP component was lacking since the only reported ACP was the SDAP subunit of the membrane-bound NADH:ubiquinone oxidoreductase, We report that most of the SDAP is found in the soluble (matrix) fraction of bovine heart mitochondria and is therefore available to carry the intermediates of type II fatty acid synthesis. (c) 2005 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

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收藏

页码：4892 / 4896

页数：5

相关论文

共 29 条

[1] Mitochondrial acyl carrier protein is involved in lipoic acid synthesis in Saccharomyces cerevisiae [J].

Brody, S ;

Oh, CK ;

Hoja, U ;

Schweizer, E .

FEBS LETTERS, 1997, 408 (02) :217-220

[2] Interaction network containing conserved and essential protein complexes in Escherichia coli [J].

Butland, G ;

Peregrín-Alvarez, JM ;

Li, J ;

Yang, WH ;

Yang, XC ;

Canadien, V ;

Starostine, A ;

Richards, D ;

Beattie, B ;

Krogan, N ;

Davey, M ;

Parkinson, J ;

Greenblatt, J ;

Emili, A .

NATURE, 2005, 433 (7025) :531-537

[3] The post-translational modifications of the nuclear encoded subunits of complex I from bovine heart mitochondria [J].

Carroll, J ;

Fearnley, IM ;

Skehel, JM ;

Runswick, MJ ;

Shannon, RJ ;

Hirst, J ;

Walker, JE .

MOLECULAR & CELLULAR PROTEOMICS, 2005, 4 (05) :693-699

[4] Analysis of the subunit composition of complex I from bovine heart mitochondria [J].

Carroll, J ;

Fearnley, IM ;

Shannon, RJ ;

Hirst, J ;

Walker, JE .

MOLECULAR & CELLULAR PROTEOMICS, 2003, 2 (02) :117-126

[5] Cloning and characterization of the lipoyl-protein ligase gene LIPB from the yeast Kluyveromyces lactis: synergistic respiratory deficiency due to mutations in LIPB and mitochondrial F-1-ATPase subunits [J].

Chen, XJ .

MOLECULAR & GENERAL GENETICS, 1997, 255 (03) :341-349

[6] Lipoyl synthase requires two equivalents of S-adenosyl-L-methionine to synthesize one equivalent of lipoic acid [J].

Cicchillo, RM ;

Iwig, DF ;

Jones, AD ;

Nesbitt, NM ;

Baleanu-Gogonea, C ;

Souder, MG ;

Tu, L ;

Booker, SJ .

BIOCHEMISTRY, 2004, 43 (21) :6378-6386

[7] IDENTIFICATION OF A NEW NUCLEAR GENE (CEM1) ENCODING A PROTEIN HOMOLOGOUS TO A BETA-KETO-ACYL SYNTHASE WHICH IS ESSENTIAL FOR MITOCHONDRIAL RESPIRATION IN SACCHAROMYCES-CEREVISIAE [J].

HARINGTON, A ;

HERBERT, CJ ;

TUNG, B ;

GETZ, GS ;

SLONIMSKI, PP .

MOLECULAR MICROBIOLOGY, 1993, 9 (03) :545-555

[8] The nuclear encoded subunits of complex I from bovine heart mitochondria [J].

Hirst, J ;

Carroll, J ;

Fearnley, IM ;

Shannon, RJ ;

Walker, JE .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2003, 1604 (03) :135-150

[9] HFA1 encoding an organelle-specific acetyl-CoA carboxylase controls mitochondrial fatty acid synthesis in Saccharomyces cerevisiae [J].

Hoja, U ;

Marthol, S ;

Hofmann, J ;

Stegner, S ;

Schulz, R ;

Meier, S ;

Greiner, E ;

Schweizer, E .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (21) :21779-21786

[10] Cloning, expression, and characterization of a human 4′-phosphopantetheinyl transferase with broad substrate specificity [J].

Joshi, AK ;

Zhang, L ;

Rangan, VS ;

Smith, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (35) :33142-33149