Parts plus pipes: Synthetic biology approaches to metabolic engineering

被引：88

作者：

Boyle, Patrick M. ^{[1
]}

Silver, Pamela A. ^{[1
,2
]}

机构：

[1] Harvard Univ, Sch Med, Dept Syst Biol, Boston, MA 02115 USA

[2] Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

来源：

METABOLIC ENGINEERING | 2012年 / 14卷 / 03期

基金：

美国国家卫生研究院;

关键词：

Synthetic biology; Metabolic engineering; Systems biology; Biological design; Biological engineering; GENE-EXPRESSION; ESCHERICHIA-COLI; PROTEIN IMPORT; SACCHAROMYCES-CEREVISIAE; TRANSCRIPTION MACHINERY; MICROBIAL-PRODUCTION; YEAST; BIOSYNTHESIS; ORGANIZATION; PATHWAY;

D O I：

10.1016/j.ymben.2011.10.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Synthetic biologists combine modular biological "parts" to create higher-order devices. Metabolic engineers construct biological "pipes" by optimizing the microbial conversion of basic substrates to desired compounds. Many scientists work at the intersection of these two philosophies, employing synthetic devices to enhance metabolic engineering efforts. These integrated approaches promise to do more than simply improve product yields; they can expand the array of products that are tractable to produce biologically. In this review, we explore the application of synthetic biology techniques to next-generation metabolic engineering challenges, as well as the emerging engineering principles for biological design. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：223 / 232

页数：10

共 129 条

[1]

Agapakis Christina M, 2010, J Biol Eng, V4, P3, DOI 10.1186/1754-1611-4-3

[2] Synthetic biology: exploring and exploiting genetic modularity through the design of novel biological networks [J].

Agapakis, Christina M. ;

Silver, Pamela A. .

MOLECULAR BIOSYSTEMS, 2009, 5 (07) :704-713

[3] Rational design of memory in eukaryotic cells [J].

Ajo-Franklin, Caroline M. ;

Drubin, David A. ;

Eskin, Julian A. ;

Gee, Elaine P. S. ;

Landgraf, Dirk ;

Phillips, Ira ;

Silver, Pamela A. .

GENES & DEVELOPMENT, 2007, 21 (18) :2271-2276

[4] Assembling materials with DNA as the guide [J].

Aldaye, Faisal A. ;

Palmer, Alison L. ;

Sleiman, Hanadi F. .

SCIENCE, 2008, 321 (5897) :1795-1799

[5] Tuning genetic control through promoter engineering [J].

Alper, H ;

Fischer, C ;

Nevoigt, E ;

Stephanopoulos, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (36) :12678-12683

[6] Global transcription machinery engineering: A new approach for improving cellular phenotype [J].

Alper, Hal ;

Stephanopoulos, Gregory .

METABOLIC ENGINEERING, 2007, 9 (03) :258-267

[7] Engineering yeast transcription machinery for improved ethanol tolerance and production [J].

Alper, Hal ;

Moxley, Joel ;

Nevoigt, Elke ;

Fink, Gerald R. ;

Stephanopoulos, Gregory .

SCIENCE, 2006, 314 (5805) :1565-1568

[8] Environmental signal integration by a modular AND gate [J].

Anderson, J. Christopher ;

Voigt, Christopher A. ;

Arkin, Adam P. .

MOLECULAR SYSTEMS BIOLOGY, 2007, 3

[9] BglBricks: A flexible standard for biological part assembly [J].

Anderson J.C. ;

Dueber J.E. ;

Leguia M. ;

Wu G.C. ;

Arkin A.P. ;

Keasling J.D. .

Journal of Biological Engineering, 4 (1)

[10] A new algorithm for RNA secondary structure design [J].

Andronescu, M ;

Fejes, AP ;

Hutter, F ;

Hoos, HH ;

Condon, A .

JOURNAL OF MOLECULAR BIOLOGY, 2004, 336 (03) :607-624

← 1 2 3 4 5 6 7 8 9 10 →