Fabrication of protein G LB film for immunoglobulin G immobilization

被引：36

作者：

Oh, BK ^{[1
]}

Chun, BS ^{[1
]}

Park, KW ^{[1
]}

Lee, WC ^{[1
]}

Lee, WH ^{[1
]}

Choi, JW ^{[1
]}

机构：

[1] Sogang Univ, Dept Chem & Biomol Engn, Seoul 121742, South Korea

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2004年 / 24卷 / 1-2期

关键词：

protein G; Langmuir-Blodgett technique; surface plasmon resonance; atomic force microscopy;

D O I：

10.1016/j.msec.2003.09.065

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

dFor the development of an immunosensor and protein chip with high efficiency, the thin film of protein G to has specific affinity to the Fc portion of immunoglobulin G (IgG) was fabricated by using the Langmuir-Blodgett (LB) technique. The formation of protein G LB film on a solid surface was confirmed by surface plasmon resonance (SPR), and the surface morphologies of the protein G LB film were observed by using atomic force microscopy (AFM). The IgG binding to protein G LB film and antigen binding to immobilized IgG were verified by SPR measurement. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：65 / 69

页数：5

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