Rapid Molecular Detection of Extrapulmonary Tuberculosis by the Automated GeneXpert MTB/RIF System

被引：293

作者：

Hillemann, Doris ^{[1
]}

Ruesch-Gerdes, Sabine ^{[1
]}

Boehme, Catharina ^{[2
]}

Richter, Elvira ^{[1
]}

机构：

[1] Forschungszentrum Borstel, Natl Referenzzentrum Mykobakterien, D-23845 Borstel, Germany

[2] FIND, Geneva, Switzerland

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 04期

关键词：

POLYMERASE-CHAIN-REACTION; MYCOBACTERIUM-TUBERCULOSIS; RIFAMPIN RESISTANCE; RPOB GENE; DIAGNOSIS; ASSAY; PCR; TECHNOLOGY;

D O I：

10.1128/JCM.02268-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In total, 521 nonrespiratory specimens (91 urine, 30 gastric aspirate, 245 tissue, 113 pleural fluid, 19 cerebrospinal fluid [CSF], and 23 stool specimens) submitted to the German National Reference Laboratory for Mycobacteria (NRL) from May 2009 to August 2010 were comparatively investigated with the new molecular-based GeneXpert MTB/RIF (Xpert) assay system and conventional liquid and solid culture methods. Twenty (3.8%) of the 521 specimens gave no interpretable result. Whereas the sensitivity of the Xpert assay with tissue specimens was 69.0% (20 out of 29 culture-positive cases detected), 100% sensitivity was found with the urine and stool specimens. The combined sensitivity and specificity of the Xpert assay were calculated to be 77.3% and 98.2%, respectively.

引用

页码：1202 / 1205

页数：4

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