Mutation analysis of CD95 (APO-1/Fas) in childhood B-lineage acute lymphoblastic leukaemia

被引:31
作者
Beltinger, C
Böhler, T
Karawajew, L
Ludwig, WD
Schrappe, M
Debatin, KM
机构
[1] Univ Ulm, Kinderklin, D-89075 Ulm, Germany
[2] Deutsch Krebsforschungszentrum, D-6900 Heidelberg, Germany
[3] Med Hsch Hannover, Abt Padiat Hamatol & Onkol, Hannover, Germany
[4] Humboldt Univ, Robert Rossle Klin, Abt Hamatol Onkol & Tumorimmunol, Berlin, Germany
关键词
CD95 (APO-1/Fas); apoptosis; B-lineage acute lymphoblastic leukaemia; mutation;
D O I
10.1046/j.1365-2141.1998.00827.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The CD95 system plays an important role in lymphocyte homeostasis, has been implicated in the development of lymphoid malignancies, exerts a tumour suppressor function, and contributes to drug-induced cytotoxicity. We hypothesized that mutations of CD95 may occur in childhood B-lineage acute lymphoblastic leukaemia (ALL), a disease known for its constitutive resistance towards CD95-mediated apoptosis. We investigated 32 primary B-lineage ALL of childhood and five B-lineage ALL cell lines. All primary leukaemias expressed CD95 and bcl-2 to a variable degree. Most of the leukaemias were resistant towards CD95-mediated apoptosis. However, using SSCP analysis, no mutations in the ceding and proximal promoter region could be detected. We conclude that the resistance towards CD95-mediated apoptosis observed in most de novo B-lineage ALL is not caused by mutations of the CD95 death receptor.
引用
收藏
页码:722 / 728
页数:7
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