High-throughput proteomics using antibody microarrays

被引：46

作者：

Wingren, C ^{[1
]}

Borrebaeck, CAK ^{[1
]}

机构：

[1] Lund Univ, Dept Immunotechnol, Lund, Sweden

来源：

EXPERT REVIEW OF PROTEOMICS | 2004年 / 1卷 / 03期

关键词：

antibody microarrays; biomarker discovery; disease diagnostics; protein expression profiling; proteome analysis; proteomics; recombinant antibody library;

D O I：

10.1586/14789450.1.3.355

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibody-based microarrays are a novel technology that hold great promise in proteomics. Microarrays can be printed with thousands of recombinant antibodies carrying the desired specificities, the biologic sample (e.g., an entire proteome) and any specifically bound analytes detected. The microarray patterns that are generated can then be converted into proteomic maps, or molecular fingerprints, revealing the composition of the proteome. Using this tool, global proteome analysis and protein expression profiling will thus provide new opportunities for biomarker discovery, drug target identification and disease diagnostics, as well as providing insights into disease biology. Intense work is currently underway to develop this novel technology platform into the high-throughput proteomic tool required by the research community.

引用

页码：355 / 364

页数：10

共 67 条

[1] Toward a human blood serum proteome - Analysis by multidimensional separation coupled with mass spectrometry [J].