Detecting parasitised eggs with polymerase chain reaction and DNA sequence of Trichogramma australicum Girault (Hymenoptera: Trichogrammatidae)

被引:17
作者
Amornsak, W [1 ]
Gordh, G
Graham, G
机构
[1] Univ Queensland, Dept Entomol, Brisbane, Qld 4072, Australia
[2] Univ Queensland, CRC Trop Pest Management, Brisbane, Qld 4072, Australia
来源
AUSTRALIAN JOURNAL OF ENTOMOLOGY | 1998年 / 37卷
关键词
DNA sequences; eggs; parasitised eggs; polymerase chain reaction; Trichogramma australicum;
D O I
10.1111/j.1440-6055.1998.tb01567.x
中图分类号
Q96 [昆虫学];
学科分类号
摘要
The nuclear internal transcribed spacer 2 (ITS2) regions of the egg parasitoid Trichogramma australicum Girault, cotton bollworm Helicoverpa armiger a (Hubner) and Helicoverpa punctigera (Wallengren) were amplified by the polymerase chain reaction (PCR) to yield 408, 588 and 644 b.p. fragments, respectively. DNA sequence information was used to design unique primers for specific amplification of T. australicum DNA from individual parasitised eggs. Helicoverpa australicum-specific PCR products were first visible 12 h after eggs were parasitised. This PCR assay can be used to monitor wasp populations under field conditions.
引用
收藏
页码:174 / 179
页数:6
相关论文
共 19 条
[11]  
SILVA IMMS, 1995, PROCEEDINGS OF THE SECTION EXPERIMENTAL AND APPLIED ENTOMOLOGY OF THE NETHERLANDS ENTOMOLOGICAL SOCIETY (N.E.V.), VOL 6, 1995, P195
[12]   EVOLUTION, WEIGHTING, AND PHYLOGENETIC UTILITY OF MITOCHONDRIAL GENE-SEQUENCES AND A COMPILATION OF CONSERVED POLYMERASE CHAIN-REACTION PRIMERS [J].
SIMON, C ;
FRATI, F ;
BECKENBACH, A ;
CRESPI, B ;
LIU, H ;
FLOOK, P .
ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA, 1994, 87 (06) :651-701
[13]  
Simon C., 1991, NATO ASI Series Series H Cell Biology, V57, P329
[14]  
TEAKLE RE, 1985, HDB INSECT REARING, V2, P313
[15]  
van Lenteren J.C., 1981, P153
[16]  
vanKan FJPM, 1996, PROCEEDINGS OF THE SECTION EXPERIMENTAL AND APPLIED ENTOMOLOGY OF THE NETHERLANDS ENTOMOLOGICAL SOCIETY (N.E.V.), VOL 7, 1996, P233
[17]  
Vanlerberghe-Masutti F., 1994, Insect Molecular Biology, V3, P229, DOI 10.1111/j.1365-2583.1994.tb00171.x
[18]  
Vanlerberghe-Masutti Flavie, 1994, Norwegian Journal of Agricultural Sciences Supplement, V16, P171
[19]   Sequence and Secondary Structure Comparisons of ITS rDNA in Mosquitoes (Diptera: Culicidae) [J].
Wesson, Dawn M. ;
Porter, Charles H. ;
Collins, Frank H. .
MOLECULAR PHYLOGENETICS AND EVOLUTION, 1992, 1 (04) :253-269