Loss of Trx-2 enhances oxidative stress-dependent phenotypes in Drosophila

被引：41

作者：

Tsuda, Manabu ^{[1
]}

Ootaka, Ryousuke ^{[1
]}

Ohkura, Chiaki ^{[1
]}

Kishita, Yoshihito ^{[1
]}

Seong, Ki-Hyeon ^{[2
]}

Matsuo, Takashi ^{[1
]}

Aigaki, Toshiro ^{[1
]}

机构：

[1] Tokyo Metropolitan Univ, Dept Biol Sci, Hachioji, Tokyo 1920397, Japan

[2] RIKEN Tsukuba Inst, Mol Genet Lab, Tsukuba, Ibaraki 3050074, Japan

来源：

FEBS LETTERS | 2010年 / 584卷 / 15期

关键词：

Thioredoxin; Paraquat; Hydrogen peroxide; Protein carbonyl; GENE SEARCH SYSTEM; LIFE-SPAN; DEADHEAD GENE; THIOREDOXIN; MITOCHONDRIAL; MELANOGASTER; OVEREXPRESSION; ATTENUATION; LONGEVITY; REDUCTASE;

D O I：

10.1016/j.febslet.2010.06.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Overexpression of thioredoxin (TRX)confers oxidative stress resistance and extends lifespan in mammals and insects. However, less is known about phenotypes associated with loss of TRX. We investigated loss-of-function phenotypes of Trx-2 in Drosophila, and found that the mutant flies are hyper-susceptible to paraquat, a free radical generator, but not to hydrogen peroxide. They contain a high amount of protein carbonyl, which dramatically increases with age. Trx-2 mutants express high levels of anti-oxidant genes, such as superoxide dismutase, catalase, and glutathione synthetase. This is the first demonstration of biochemical and physiological consequences caused by loss of Trx-2 in Drosophila. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3398 / 3401

页数：4

共 26 条

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