Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection

被引：19

作者：

Craig, DB ^{[1
]}

Polakowski, PM ^{[1
]}

Arriaga, E ^{[1
]}

Wong, JCY ^{[1
]}

Ahmadzadeh, H ^{[1
]}

Stathakis, C ^{[1
]}

Dovichi, NJ ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada

来源：

ELECTROPHORESIS | 1998年 / 19卷 / 12期

关键词：

protein separation; sieving matrix; sodium dodecyl sulfate; capillary electrophoresis;

D O I：

10.1002/elps.1150191222

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a method for protein labeling, separation by capillary electrophoresis in a polymer sieving matrix, and detection by laser-induced fluorescence. Different dyes are used to label standard and sample proteins. A two-spectral channel detector resolves fluorescence from the sample and standards. Comparison of the migration time of the sample and standards permits the precise determination of molecular weight, irrespective of variations in run-to-run migration times.

引用

页码：2175 / 2178

页数：4