Overlapping deletions spanning the proximal two-thirds of the mouse t complex

被引:11
作者
Bergstrom, DE [1 ]
Bergstrom, RA [1 ]
Munroe, RJ [1 ]
Lee, BK [1 ]
Browning, VL [1 ]
You, Y [1 ]
Eicher, EM [1 ]
Schimenti, JC [1 ]
机构
[1] Jackson Lab, Bar Harbor, ME 04609 USA
关键词
D O I
10.1007/s00335-003-2298-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chromosome deletion complexes in model organisms serve as valuable genetic tools for the functional and physical annotation of complex genomes. Among their many roles, deletions can serve as mapping tools for simple or quantitative trait loci (QTLs), genetic reagents for regional mutagenesis experiments, and, in the case of mice, models of human contiguous gene deletion syndromes. Deletions also are uniquely suited for identifying regions of the genome containing haploinsufficient or imprinted loci. Here we describe the creation of new deletions at the proximal end of mouse Chromosome (Chr) 17 by using the technique of ES cell irradiation and the extensive molecular characterization of these and previously isolated deletions that, in total, cover much of the mouse t complex. The deletions are arranged in five overlapping complexes that collectively span about 25 Mbp. Furthermore, we have integrated each of the deletion complexes with physical data from public and private mouse genome sequences, and our own genetic data, to resolve some discrepancies. These deletions will be useful for characterizing several phenomena related to the t complex and t haplotypes, including transmission ratio distortion, male infertility, and the collection of t haplotype embryonic lethal mutations. The deletions will also be useful for mapping other loci of interest on proximal Chr 17, including T-associated sex reversal (Tas) and head-tilt (het). The new deletions have thus far been used to localize the recently identified t haplolethal (Thl1) locus to an approximately 1.3-Mbp interval.
引用
收藏
页码:817 / 829
页数:13
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  • [2] DELETION ANALYSIS OF MALE-STERILITY EFFECTS OF TERT-HAPLOTYPES IN THE MOUSE
    BENNETT, D
    ARTZT, K
    [J]. GENETICAL RESEARCH, 1990, 56 (2-3) : 179 - 183
  • [3] T-LOCUS OF MOUSE
    BENNETT, D
    [J]. CELL, 1975, 6 (04) : 441 - 454
  • [4] Bergstrom RA, 1998, GENETICS, V150, P815
  • [5] A new spontaneous deletion on Chromosome 17 including brachyury
    Bilinski, P
    Schimenti, J
    Gossler, A
    [J]. MAMMALIAN GENOME, 1997, 8 (12) : 932 - 933
  • [6] Browning VL, 2002, GENETICS, V160, P675
  • [7] Davis AP, 1998, GENETICS, V148, P7
  • [8] A microsatellite map of the pink-eyed dilution (p) deletion complex in mouse Chromosome 7
    Dhar, MS
    Johnson, DK
    [J]. MAMMALIAN GENOME, 1997, 8 (02) : 143 - 145
  • [9] A comprehensive genetic map of the mouse genome
    Dietrich, WF
    Miller, J
    Steen, R
    Merchant, MA
    DamronBoles, D
    Husain, Z
    Dredge, R
    Daly, MJ
    Ingalls, KA
    OConnor, TJ
    Evans, CA
    DeAngelis, MM
    Levinson, DM
    Kruglyak, L
    Goodman, N
    Copeland, NG
    Jenkins, NA
    Hawkins, TL
    Stein, L
    Page, DC
    Lander, ES
    [J]. NATURE, 1996, 380 (6570) : 149 - 152
  • [10] DellBank: a mouse ES-cell resource for generating deletions
    Goodwin, NC
    Ishida, Y
    Hartford, S
    Wnek, C
    Bergstrom, RA
    Leder, P
    Schimenti, JC
    [J]. NATURE GENETICS, 2001, 28 (04) : 310 - 311