Synaptic Homeostasis and Restructuring across the Sleep-Wake Cycle

被引：36

作者：

Blanco, Wilfredo ^{[1
,2
,3
]}

Pereira, Catia M. ^{[4
]}

Cota, Vinicius R. ^{[1
,5
]}

Souza, Annie C. ^{[1
]}

Renno-Costa, Cesar ^{[1
]}

Santos, Sharlene ^{[1
]}

Dias, Gabriella ^{[1
]}

Guerreiro, Ana M. G. ^{[6
]}

Tort, Adriano B. L. ^{[1
]}

Neto, Adriao D. ^{[2
]}

Ribeiro, Sidarta ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Norte, Inst Brain, BR-59072970 Natal, RN, Brazil

[2] Univ Fed Rio Grande do Norte, Dept Comp & Automat, BR-59072970 Natal, RN, Brazil

[3] State Univ Rio Grande do Norte, Dept Comp Sci, Natal, RN, Brazil

[4] ELS IINN, Natal, RN, Brazil

[5] Univ Fed Sao Joao del Rei, Lab Neuroengn & Neurosci, Sao Joao Del Rei, MG, Brazil

[6] Univ Fed Rio Grande do Norte, Dept Biomed Engn, Natal, RN, Brazil

来源：

PLOS COMPUTATIONAL BIOLOGY | 2015年 / 11卷 / 05期

关键词：

LONG-TERM POTENTIATION; SLOW-WAVE SLEEP; MEMORY CONSOLIDATION; GENE-EXPRESSION; HIPPOCAMPAL-NEURONS; DENDRITIC SPINES; RAT HIPPOCAMPUS; ENHANCES MEMORY; PLASTICITY; ACTIVATION;

D O I：

10.1371/journal.pcbi.1004241

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Sleep is critical for hippocampus-dependent memory consolidation. However, the underlying mechanisms of synaptic plasticity are poorly understood. The central controversy is on whether long-term potentiation (LTP) takes a role during sleep and which would be its specific effect on memory. To address this question, we used immunohistochemistry to measure phosphorylation of Ca2+/calmodulin-dependent protein kinase II (pCaMKIIa) in the rat hippocampus immediately after specific sleep-wake states were interrupted. Control animals not exposed to novel objects during waking (WK) showed stable pCaMKIIa levels across the sleep-wake cycle, but animals exposed to novel objects showed a decrease during subsequent slow-wave sleep (SWS) followed by a rebound during rapid-eye-movement sleep (REM). The levels of pCaMKIIa during REM were proportional to cortical spindles near SWS/REM transitions. Based on these results, we modeled sleep-dependent LTP on a network of fully connected excitatory neurons fed with spikes recorded from the rat hippocampus across WK, SWS and REM. Sleep without LTP orderly rescaled synaptic weights to a narrow range of intermediate values. In contrast, LTP triggered near the SWS/REM transition led to marked swaps in synaptic weight ranking. To better understand the interaction between rescaling and restructuring during sleep, we implemented synaptic homeostasis and embossing in a detailed hippocampal-cortical model with both excitatory and inhibitory neurons. Synaptic homeostasis was implemented by weakening potentiation and strengthening depression, while synaptic embossing was simulated by evoking LTP on selected synapses. We observed that synaptic homeostasis facilitates controlled synaptic restructuring. The results imply a mechanism for a cognitive synergy between SWS and REM, and suggest that LTP at the SWS/REM transition critically influences the effect of sleep: Its lack determines synaptic homeostasis, its presence causes synaptic restructuring.

引用

页数：29

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