Cellobiohydrolase I as a chiral additive in capillary electrophoresis and liquid chromatography

Cellobiohydrolase I(CBH I) was used as a chiral selector in free solution in capillary electrophoresis, applying the partial filling technique. The enantiomers of the amino alcohols oxprenolol and propranolol could be completely resolved with selector plugs of only 1.0 cm in plain acetate buffer at pH 5.0. Although warfarin migrates in the same direction as CBH I at this pH, its enantiomers were separated and detected at the anodic end of the capillary, well resolved from the protein plug. Furthermore, propranolol was enantioseparated at pH 3.0, where the protein has a positive net charge and migrates in the same direction as this amino alcohol. Addition of 2-propanol significantly improved the peak shape of overloaded peaks. CBH I was also used as a complexing agent in the mobile phase in HPLC for chiral separation of rac-propranolol. (C) 1998 Elsevier Science B.V. All rights reserved.