Directed molecular evolution in plant improvement

被引：37

作者：

Lassner, M ^{[1
]}

Bedbrook, J ^{[1
]}

机构：

[1] Maxygen Inc, Redwood City, CA 94063 USA

来源：

CURRENT OPINION IN PLANT BIOLOGY | 2001年 / 4卷 / 02期

关键词：

D O I：

10.1016/S1369-5266(00)00152-7

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Directed molecular evolution is a powerful tool to evolve genes with commercial applications. Its most common application is to evolve enzymes with improved kinetics, altered substrate or product specificities, or improved function in different cellular environments. The technique is beginning to be applied to goals relevant to agriculture. Recent examples include the generation of novel carotenoids, enhanced herbicide detoxification, and the improvement of insect resistance genes.

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页码：152 / 156

页数：5

相关论文

共 20 条

[1] Novel hydroxycarotenoids with improved antioxidative properties produced by gene combination in Escherichia coli [J].

Albrecht, M ;

Takaichi, S ;

Steiger, S ;

Wang, ZY ;

Sandmann, G .

NATURE BIOTECHNOLOGY, 2000, 18 (08) :843-846

[2] Design by directed evolution [J].

Arnold, FH .

ACCOUNTS OF CHEMICAL RESEARCH, 1998, 31 (03) :125-131

[3]

CALDWELL RC, 1992, PCR METH APPL, V2, P28

[4] TUNING THE ACTIVITY OF AN ENZYME FOR UNUSUAL ENVIRONMENTS - SEQUENTIAL RANDOM MUTAGENESIS OF SUBTILISIN-E FOR CATALYSIS IN DIMETHYLFORMAMIDE [J].

CHEN, KQ ;

ARNOLD, FH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (12) :5618-5622

[5] Molecular evolution of an arsenate detoxification pathway DNA shuffling [J].

Crameri, A ;

Dawes, G ;

Rodriguez, E ;

Silver, S ;

Stemmer, WPC .

NATURE BIOTECHNOLOGY, 1997, 15 (05) :436-438

[6] DNA shuffling of a family of genes from diverse species accelerates directed evolution [J].

Crameri, A ;

Raillard, SA ;

Bermudez, E ;

Stemmer, WPC .

NATURE, 1998, 391 (6664) :288-291

[7]

Dannert CS, 2000, NAT BIOTECHNOL, V18, P750

[8] NUCLEOTIDE-SEQUENCE OF CRYSTAL PROTEIN GENE ISOLATED FROM B-THURINGIENSIS SUBSPECIES ENTOMOCIDUS 605 CODING FOR A TOXIN HIGHLY-ACTIVE AGAINST SPODOPTERA SPECIES [J].

HONEE, G ;

VANDERSALM, T ;

VISSER, B .

NUCLEIC ACIDS RESEARCH, 1988, 16 (13) :6240-6240

[9] Directed evolution of the surface chemistry of the reporter enzyme β-glucuronidase [J].

Matsumura, I ;

Wallingford, JB ;

Surana, NK ;

Vize, PD ;

Ellington, AD .

NATURE BIOTECHNOLOGY, 1999, 17 (07) :696-701

[10] Protein evolution by molecular breeding [J].

Minshull, J ;

Stemmer, WPC .

CURRENT OPINION IN CHEMICAL BIOLOGY, 1999, 3 (03) :284-290