Nodule formation and mineralisation of human primary osteoblasts cultured on a porous bioactive glass scaffold

被引：326

作者：

Gough, JE

Jones, JR

Hench, LL

机构：

[1] UMIST, Manchester Mat Sci Ctr, Manchester M1 7HS, Lancs, England

[2] Univ London Imperial Coll Sci Technol & Med, Dept Mat, London SW7 2BP, England

来源：

BIOMATERIALS | 2004年 / 25卷 / 11期

基金：

英国医学研究理事会;

关键词：

osteoblast; bioactive glass; ion release; mineralisation; apoptosis;

D O I：

10.1016/j.biomaterials.2003.07.001

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The aim of this study was to analyse human osteoblast responses to a porous bioactive glass scaffold. It was hypothesised that osteoblasts would attach, proliferate and form mineralised nodules in response to culture on the bioactive glass. As dissolution products are a key feature of bioactive glasses, this was measured by inductively coupled plasma optical emission spectroscopy to determine effects of both the glass surface and ion release. Osteoblasts attached and proliferated on the foams as demonstrated by scanning electron microscopy. Nodule formation was also observed in the pores of the glass and also in conditioned medium containing dissolution products at certain concentrations and these nodules were shown to be mineralised by alizarin red staining. Undiluted dissolution products from the foams however caused significant apoptosis suggesting an ion concentration dependent response. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：2039 / 2046

页数：8

共 37 条

[31] Interactions of bioactive glasses with osteoblasts in vitro:: effects of 45S5 Bioglass®, and 58S and 77S bioactive glasses on metabolism, intracellular ion concentrations and cell viability [J].