Crystallization and preliminary X-ray study of pig liver dihydropyrimidine dehydrogenase

被引:7
作者
Dobritzsch, D [1 ]
Persson, K [1 ]
Schneider, G [1 ]
Lindqvist, Y [1 ]
机构
[1] Karolinska Inst, Dept Med Biochem & Biophys, Stockholm, Sweden
来源
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2001年 / 57卷
关键词
D O I
10.1107/S0907444900015250
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Dihydropyrimidine dehydrogenase catalyzes the first and rate-limiting reaction in pyrimidine catabolism. The enzyme contains one FMN, one FAD and four Fe-S clusters per subunit of 1025 amino acids as prosthetic groups. It is also the major determinant of bioavailability and toxicity of 5-fluorouracil, a chemotherapeutic agent widely used in the treatment of solid tumors. Crystals of this enzyme diffracting to at least 2.5 Angstrom have been obtained by the hanging-drop vapour-diffusion method and belong to space group P2(1) (unit-cell parameters a = 82.0, b = 159.3, c = 163.6 Angstrom, beta = 96.1 degrees), with two homodimers per asymmetric unit.
引用
收藏
页码:153 / 155
页数:3
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