The mouse homolog of HEN1 is a potential methylase for Piwi-interacting RNAs

被引：132

作者：

Kirino, Yohei ^{[1
]}

Mourelatos, Zissimos ^{[1
]}

机构：

[1] Univ Penn, Sch Med, Dept Pathol & Lab Med, Div Neuropathol, Philadelphia, PA 19104 USA

来源：

RNA | 2007年 / 13卷 / 09期

关键词：

piRNA; piwi; miwi; mili; miRNA; microRNA; argonaute;

D O I：

10.1261/rna.659307

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Piwi-interacting RNAs (piRNAs) are a novel class of small regulatory RNAs that are expressed specifically and abundantly in germ cells. Mammalian piRNAs are 26-31 nucleotides in length and bind to Piwi proteins, but their function and biogenesis remain elusive. We previously showed that mammalian piRNAs are 2 '-O-methylated at their 39 termini. The biosynthetic mechanism and function of this modification is unknown. Here, we report that the mouse homolog (mHEN1) of HEN1, a plant microRNA ( miRNA) 2 '-O-methyltransferase, is expressed specifically in testis and methylates 3 ' termini of piRNAs in vitro. These findings provide insight into the biogenesis of piRNAs.

引用

页码：1397 / 1401

页数：5

共 24 条

[1] The small RNA profile during Drosophila melanogaster development [J].

Aravin, AA ;

Lagos-Quintana, M ;

Yalcin, A ;

Zavolan, M ;

Marks, D ;

Snyder, B ;

Gaasterland, T ;

Meyer, J ;

Tuschl, T .

DEVELOPMENTAL CELL, 2003, 5 (02) :337-350

[2] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[3]

Brennecke J, 2007, CELL, V128, P1089, DOI 10.1016/j.cell.2007.01.043

[4] The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis [J].

Carmell, MA ;

Xuan, ZY ;

Zhang, MQ ;

Hannon, GJ .

GENES & DEVELOPMENT, 2002, 16 (21) :2733-2742

[5] RNA interference is mediated by 21-and 22-nucleotide RNAs [J].

Elbashir, SM ;

Lendeckel, W ;

Tuschl, T .

GENES & DEVELOPMENT, 2001, 15 (02) :188-200

[6] A slicer-mediated mechanism for repeat-associated siRNA 5′ end formation in Drosophila [J].

Gunawardane, Lalith S. ;

Saito, Kuniaki ;

Nishida, Kazumichi M. ;

Miyoshi, Keita ;

Kawamura, Yoshinori ;

Nagami, Tomoko ;

Siomi, Haruhiko ;

Siomi, Mikiko C. .

SCIENCE, 2007, 315 (5818) :1587-1590

[7]

HORWICH MD, 2007, CURR BIOL

[8] A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in zebrafish [J].

Houwing, Saskia ;

Kamminga, Leonie M. ;

Berezikov, Eugene ;

Cronembold, Daniela ;

Girard, Angelique ;

van den Elst, Hans ;

Filippov, Dmitri V. ;

Blaser, Heiko ;

Raz, Erez ;

Moens, Cecilia B. ;

Plasterk, Ronald H. A. ;

Hannon, Gregory J. ;

Draper, Bruce W. ;

Ketting, Rene F. .

CELL, 2007, 129 (01) :69-82

[9] A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA [J].

Hutvágner, G ;

McLachlan, J ;

Pasquinelli, AE ;

Bálint, É ;

Tuschl, T ;

Zamore, PD .

SCIENCE, 2001, 293 (5531) :834-838

[10] Small RNAs just got bigger: Piwi-interacting RNAs (piRNAs) in mammalian testes [J].

Kim, V. Narry .

GENES & DEVELOPMENT, 2006, 20 (15) :1993-1997

← 1 2 3 →