Androgen-sensitive human prostate cancer cells, LNCaP, produce both N-terminally mature and truncated prostate-specific antigen isoforms

被引:23
作者
Herrala, A
Kurkela, R
Vihinen, M
Kalkkinen, N
Vihko, P
机构
[1] Oulu Univ, Bioctr, FIN-90220 Oulu, Finland
[2] Oulu Univ, WHO, Collaborating Ctr Res Reprod Hlth, FIN-90220 Oulu, Finland
[3] Univ Helsinki, Dept Biosci, Div Biochem, FIN-00014 Helsinki, Finland
[4] Tampere Univ, Inst Med Technol, FIN-33101 Tampere, Finland
[5] Univ Helsinki, Inst Biotechnol, Prot Chem Lab, FIN-00014 Helsinki, Finland
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1998年 / 255卷 / 02期
关键词
prostate cancer; N-terminal sequencing; molecular modeling; standardization; enzyme activity;
D O I
10.1046/j.1432-1327.1998.2550329.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To characterize prostate-specific antigen (PSA) produced by cancer cells, different isoforms of PSA secreted by the human prostate cancer cells, LNCaP, were purified. LNCaP-PSA production was induced by synthetic androgen, R1881. LNCaP-PSA was separated into four pools. The molecular mass of LNCaP-PSA isoforms in these pools was 34 kDa under reducing conditions and 29 kDa under nonreducing conditions on SDS/PAGE. pI of LNCaP-PSA isoforms varied from 6.8 to 8.2. Pool A had the highest specific activity, 37 nmol/(minxmg). All the pools formed stable complexes with alpha 1-antichymotrypsin and alpha 2-macroglobulin. The Fools contained 10-60% of N-terminally correctly processed LNCaP-PSA isoforms. According to the molecular modelling, the addition or deletion of two or four N-terminal amino acids could affect the three-dimensional structure and thereby remarkably reduce the enzyme activity of LNCaP-PSA.
引用
收藏
页码:329 / 335
页数:7
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