Preparative isolation and purification of cuminaldehyde and p-menta-1,4-dien-7-al from the essential oil of Cuminum cyminum L. by high-speed counter-current chromatography

被引：45

作者：

Chen, Qinqin ^{[1
]}

Hu, Xuefang ^{[1
]}

Li, Jingming ^{[1
]}

Liu, Ping ^{[1
]}

Yang, Yang ^{[1
]}

Ni, Yuanying ^{[1
]}

机构：

[1] China Agr Univ, Coll Food Sci & Nutr Engn, Key Lab Fruits & Vegetables Proc,Minist Agr, Engn Res Ctr Fruits & Vegetables Proc,Minist Educ, Beijing 100083, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2011年 / 689卷 / 01期

关键词：

High-speed counter-current chromatography (HSCCC); Cumin essential oil; Cuminaldehyde; p-Menta-1,4-dien-7-al; CHEMICAL-CONSTITUENTS; SEEDS; ANTIOXIDANT; COMPONENTS; SEPARATION; SPICES; LEAVES; PLANT; ACID; ROOT;

D O I：

10.1016/j.aca.2011.01.038

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

High-speed counter-current chromatography (HSCCC) technique in semi-preparative scale was successfully used in isolation and purification of cuminaldehyde and p-menta-1,4-dien-7-al from the essential oil of Cuminum cyminum L by using a two-phase solvent system composed of n-hexane-methanol-water (5:4:1, v/v/v). The targeted compounds were isolated, collected, purified by HSCCC in the head-tail mode, and then analyzed by gas chromatography (GC). A total of 12.72 +/- 0.22 mg of cuminaldehyde and 10.61 +/- 0.27 mg of p-menta-1,4-dien-7-al were obtained from 50 mg of the essential oil of C. cyminum L in less than 6h, with purities of 95.42% and 97.21%, respectively. In addition to GC-EI/MS, the identity of the cuminaldehyde was further confirmed with the retention time using the method of standard addition, while, the structural identification of p-menta-1,4-dien-7-al was performed with GC-EI/MS, H-1 NMR and H-1-H-1 COSY. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：149 / 154

页数：6

共 34 条

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Antimicrobial Property, Antioxidant Capacity, and Cytotoxicity of Essential Oil from Cumin Produced in Iran [J].