Isolation and purification of three flavonoid glycosides from the leaves of Nelumbo nucifera (Lotus) by high-speed counter-current chromatography

被引：95

作者：

Deng, Shengguo ^{[1
]}

Deng, Zeyuan ^{[1
]}

Fan, Yawei ^{[1
]}

Peng, You ^{[1
]}

Li, Jing ^{[1
]}

Xiong, Dongmei ^{[1
]}

Liu, Rong ^{[1
]}

机构：

[1] Nanchang Univ, Inst Adv Study, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 24期

关键词：

Counter-current chromatography; Flavonoid glycosides; Isoquercitrin; Hyperoside; Astragalin; PREPARATIVE ISOLATION; DIETARY FLAVONOIDS; EXTRACT; CELLS; ROOT;

D O I：

10.1016/j.jchromb.2009.06.026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of flavonoid glycosides from the leaves of Nelumbo nucifera (Lotus) by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (1:51:5, v/v/v/v). The targeted compounds isolated, collected and purified by HSCCC were analyzed by high performance liquid chromatography (HPLC). A total of 4.6 mg of isoquercitrin, 9.1 mg of hyperoside and 3.0 mg of astragalin with the purity of 95.8%, 97.5% and 98.3%, respectively, were obtained in one-step separation and less than 6 h from 80 mg of crude extract from the leaves of N. nucifera. The chemical structures of all the three compounds were identified by MS. H-1 NMR, C-13 NMR. Astragalin was obtained from N. nucifera for the first time. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：2487 / 2492

页数：6

共 24 条

[1] QUERCETIN EXERTS A PREFERENTIAL CYTOTOXIC EFFECT ON ACTIVE DIVIDING COLON-CARCINOMA HT29 AND CACO-2 CELLS [J].