Positional assembly of enzymes in polymersome nanoreactors for cascade reactions

被引：360

作者：

Vriezema, Dennis M.

Garcia, Paula M. L.

Oltra, Nuria Sancho

Hatzakis, Nikos S.

Kuiper, Suzanne M.

Nolte, Roeland J. M.

Rowan, Alan E.

van Hest, Jan C. M.

机构：

[1] NV Organon, Dept Med Chem, NL-5342 CC Oss, Netherlands

[2] Radboud Univ Nijmegen, Dept Organ Chem, Inst Mol & Mat, NL-6525 ED Nijmegen, Netherlands

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 39期

关键词：

cascade reactions; catalysis; enzymes; polymersomes; supramolecular chemistry;

D O I：

10.1002/anie.200701125

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In a good position: Nanoreactors can be constructed by the controlled positioning of glucose oxidase (GOX) and horseradish peroxidase (HRP) within the central water pool and block-copolymer membrane of polymersomes. A one-pot multistep reaction sequence is performed with the nanoreactor in combination with free Candida antarctica lipase B (CALB) in the bulk solution (see picture; ABTS: 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)). (Chemical Equation Presented) © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：7378 / 7382

页数：5

共 23 条

[1]

[Anonymous], 2003, ANGEW CHEM

[2]

Avnir D, 2006, J MATER CHEM, V16, P1013, DOI 10.1039/5512706h

[3] Artificial organelle: ATP synthesis from cellular mimetic polymersomes [J].