Pyrosequencing-based approach for rapid detection of rifampin-resistant Mycobacterium tuberculosis

被引：25

作者：

Zhao, JR ^{[1
]}

Bai, YJ

Zhang, QH

Wang, Y

Luo, M

Yan, XJ

机构：

[1] Fourth Mil Med Univ, Inst Genet Diag, Xian 710032, Peoples R China

[2] Xian Tuberculosis Hosp, Clin Lab, Xian 710061, Peoples R China

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2005年 / 51卷 / 02期

关键词：

Mycobacterium tuberculosis; rifampin resistance; polymerase chain reaction; pyrosequencing;

D O I：

10.1016/j.diagmicrobio.2004.10.001

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Rifampin resistance in Mycobacterium tuberculosis could be determined within 2 h by using pyrosequencing-based approach. Pyrosequencing results of rifampin-resistant (n = 21) and rifampin-susceptible (n = 20) M. tuberculosis isolates were concordant with those based on drug susceptibility testing and conventional DNA sequencing. Results showed pyrosequencing-based approach is a rapid, sensitive, and efficient detection method of rifampin-resistant M. tuberculosis. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：135 / 137

页数：3

共 17 条

[1] Single-nucleotide polymorphism analysis by pyrosequencing [J].

Ahmadian, A ;

Gharizadeh, B ;

Gustafsson, AC ;

Sterky, F ;

Nyrén, P ;

Uhlén, M ;

Lundeberg, J .

ANALYTICAL BIOCHEMISTRY, 2000, 280 (01) :103-110

[2] Characterization of rpoB mutations in rifampin-resistant clinical isolates of Mycobacterium tuberculosis from turkey by DNA sequencing and line probe assay [J].

Cavusoglu, C ;

Hilmioglu, S ;

Guneri, S ;

Bilgic, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (12) :4435-4438

[3]

Ehn M, 2002, ELECTROPHORESIS, V23, P3289, DOI 10.1002/1522-2683(200210)23:19<3289::AID-ELPS3289>3.0.CO

[4]

2-#

[5] Rapid detection of rpoB gene mutations in rifampin-resistant Mycobacterium tuberculosis isolates in Shanghai by using the amplification refractory mutation system [J].

Fan, XY ;

Hu, ZY ;

Xu, FH ;

Yan, ZQ ;

Guo, SQ ;

Li, ZM .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (03) :993-997

[6] Mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis strains isolated mostly in Asian countries and their rapid detection by line probe assay [J].

Hirano, K ;

Abe, C ;

Takahashi, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (08) :2663-2666

[7] CHARACTERIZATION BY AUTOMATED DNA-SEQUENCING OF MUTATIONS IN THE GENE (RPOB) ENCODING THE RNA-POLYMERASE BETA-SUBUNIT IN RIFAMPIN-RESISTANT MYCOBACTERIUM-TUBERCULOSIS STRAINS FROM NEW-YORK-CITY AND TEXAS [J].

KAPUR, V ;

LI, LL ;

IORDANESCU, S ;

HAMRICK, MR ;

WANGER, A ;

KREISWIRTH, BN ;

MUSSER, JM .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (04) :1095-1098

[8] Pyrosequencing technology as a method for the diagnosis of multiple endocrine neoplasia type 2 [J].

Kruckeberg, KE ;

Thibodeau, SN .

CLINICAL CHEMISTRY, 2004, 50 (03) :522-529

[9] Detection of rifampin resistance in Mycobacterium tuberculosis by use of a rapid, simple, and specific RNA/RNA mismatch assay [J].

Nash, KA ;

Gaytan, A ;

Inderlied, CB .

JOURNAL OF INFECTIOUS DISEASES, 1997, 176 (02) :533-536

[10] Improved performance of pyrosequencing using single-stranded DNA-binding protein [J].

Ronaghi, M .

ANALYTICAL BIOCHEMISTRY, 2000, 286 (02) :282-288

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