Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis

被引：47

作者：

Ashelford, Kevin ^{[1
]}

Eriksson, Maria E. ^{[2
]}

Allen, Christopher M. ^{[3
]}

D'Amore, Rosalinda ^{[1
]}

Johansson, Mikael ^{[2
]}

Gould, Peter ^{[1
]}

Kay, Suzanne ^{[1
]}

Millar, Andrew J. ^{[4
]}

Hall, Neil ^{[1
]}

Hall, Anthony ^{[1
]}

机构：

[1] Univ Liverpool, Sch Biol Sci, Liverpool L69 7ZB, Merseyside, England

[2] Umea Univ, Umea Plant Sci Ctr, Dept Plant Physiol, SE-90187 Umea, Sweden

[3] Appl Biosyst Inc, Warrington WA3 7QH, Cheshire, England

[4] Univ Edinburgh, Sch Biol Sci, Inst Mol Plant Sci, Edinburgh EH9 3JH, Midlothian, Scotland

来源：

GENOME BIOLOGY | 2011年 / 12卷 / 03期

基金：

英国生物技术与生命科学研究理事会; 瑞典研究理事会; 英国工程与自然科学研究理事会;

关键词：

GENETIC-ANALYSIS; KEY PATHWAYS; THALIANA; IDENTIFICATION; EXPRESSION; PROTEIN; POLYMORPHISMS; TRANSCRIPTION; MUTANTS; ENCODES;

D O I：

10.1186/gb-2011-12-3-r28

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.

引用

页数：12

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