An electrochemiluminescent biosensor for uric acid based on the electrochemiluminescence of bis-[3,4,6-trichloro-2-(pentyloxycarbonyl)-phenyl] oxalate on an ITO electrode modified by an electropolymerized nickel phthalocyanine film

被引：23

作者：

Lin, Zhenyu ^{[1
,2
]}

Chen, Zhihuang ^{[1
,2
]}

Liu, Yan ^{[1
,2
]}

Wang, Jian ^{[1
,2
]}

Chen, Guonan ^{[1
,2
]}

机构：

[1] Fuzhou Univ, Dept Chem, Fuzhou 350002, Peoples R China

[2] Fuzhou Univ, Dept Chem, Minist Educ, Key Lab Anal & Detect Technol Food Safety, Fuzhou 350002, Peoples R China

来源：

ANALYST | 2008年 / 133卷 / 06期

关键词：

D O I：

10.1039/b716281b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nickel(II) tetrasulfophthalo-cyanine (NiTSPc) could be electrodeposited onto an indium tin oxide (ITO) electrode to form an electropolymerization film of NiTSPc. The electrochemiluminescent (ECL) behavior of bis-[3,4,6-trichloro-2-(pentyloxy-carbonyl)-phenyl] oxalate (BTPPO) on this modified electrode was investigated in detail. The emission of ECL of BTPPO can be greatly enhanced by hydrogen peroxide at this modified electrode. Thus, a new ECL biosensor for uric acid was developed based on the enzymatic reaction of uric acid in the presence of uricase to produce H2O2. The developed sensor has been used to detect uric acid in real serum samples, and the results compared well with those obtained by the routine method.

引用

页码：797 / 801

页数：5

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