A new electrochemiluminescent sensing system for glucose based on the electrochemilumine scent reaction of bis-[3,4,6-trichloro-2-(pentyloxycarbonyl)-phenyl] oxalate

In this paper, the electrochemiluminescence (ECL) behavior of bis-[3,4,6-trichloro-2-(pentyloxycarbonyl)-phenyl] oxalate (BTPPO) at glassy carbon electrode (GCE) in phosphate buffer solution in the presence of hydrogen peroxide has been investigated when linear sweep voltammetry was applied. The optimum chemical conditions and electrochemical parameters for this ECL system have been investigated in detail. Under the optimum conditions, it was found that the concentration of BTPPO was linear with the ECL intensity in the range of 3.0 x 10(-6) to 3.0 x 10-(4) mol/L, and the detection limit (S/N = 3) for BTPPO was 1.0 x 10(-7) mol/L. The possible mechanism for ECL of BTPPO at the GCE in the presence of hydrogen peroxide was also discussed. Furthermore, based on the fact that glucose oxidase can react with glucose to produce hydrogen peroxide, a new ECL sensing system of BTPPO has been developed for detection of glucose. The enhanced ECL intensity has a linear relationship with the concentration of glucose in the range of 1.0 x 10(-4) to 1.0 x 10(-3) mol/L, and the detection limit for glucose is found to be 5.0 x 10(-5) mol/L (S/N = 3). (C) 2007 Elsevier B.V. All rights reserved.