Abscisic acid determines arbuscule development and functionality in the tomato arbuscular mycorrhiza

被引:212
作者
Herrera-Medina, Maria Jose
Steinkellner, Siegrid
Vierheilig, Horst
Bote, Juan Antonio Ocampo
Garrido, Jose Manuel Garcia
机构
[1] CSIC, Dept Soil Microbiol & Symbiosis Syst, Estac Expt Zaidin, E-18008 Granada, Spain
[2] Univ Nat Resources & Appl Life Sci, Inst Pflanzenschutz, Dept Angew Pflanzenwiss & Pflanzenbiotech, A-1190 Vienna, Austria
关键词
abscisic acid; arbuscular mycorrhiza; ethylene; sitiens; tomato (Lycopersicon esculentum);
D O I
10.1111/j.1469-8137.2007.02107.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The role of abscisic acid (ABA) during the establishment of the arbuscular mycorrhiza (AM) was studied using ABA sitiens tomato (Lycopersicon esculentum) mutants with reduced ABA concentrations. Sitiens plants and wild-type (WT) plants were colonized by Glomus intraradices. Trypan blue and alkaline phosphatase histochemical staining procedures were used to determine both root colonization and fungal efficiency. Exogenous ABA and silver thiosulfate (STS) were applied to establish the role of ABA and putative antagonistic cross-talk between ABA and ethylene during AM formation, respectively. Sitiens plants were less susceptible to the AM fungus than WT plants. Microscopic observations and arbuscule quantification showed differences in arbuscule morphology between WT and sitiens plants. Both ABA and STS increased susceptibility to the AM fungus in WT and sitiens plants. Fungal alkaline phosphate activity in sitiens mutants was completely restored by ABA application. The results demonstrate that ABA contributes to the susceptibility of tomato to infection by AM fungi, and that it seems to play an important role in the development of the complete arbuscule and its functionality. Ethylene perception is crucial to AM regulation, and the impairment of mycorrhiza development in ABA-deficient plants is at least partly attributable to ethylene.
引用
收藏
页码:554 / 564
页数:11
相关论文
共 43 条
[1]   PHYTOHORMONE CHANGES IN BOUTELOUA-GRACILIS INFECTED BY VESICULAR ARBUSCULAR MYCORRHIZAE .2. ALTERED LEVELS OF GIBBERELLIN-LIKE SUBSTANCES AND ABSCISIC-ACID IN THE HOST PLANT [J].
ALLEN, MF ;
MOORE, TS ;
CHRISTENSEN, M .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1982, 60 (04) :468-471
[2]   Antagonistic interaction between abscisic acid and jasmonate-ethylene signaling pathways modulates defense gene expression and disease resistance in Arabidopsis [J].
Anderson, JP ;
Badruzsaufari, E ;
Schenk, PM ;
Manners, JM ;
Desmond, OJ ;
Ehlert, C ;
Maclean, DJ ;
Ebert, PR ;
Kazan, K .
PLANT CELL, 2004, 16 (12) :3460-3479
[3]  
[Anonymous], 1992, MOL SIGNAL PLANT MIC
[4]  
Audenaert K, 2002, PLANT PHYSIOL, V128, P491, DOI 10.1104/pp.010605
[5]   Interactions between abscisic acid and ethylene signaling cascades [J].
Beaudoin, N ;
Serizet, C ;
Gosti, F ;
Giraudat, J .
PLANT CELL, 2000, 12 (07) :1103-1115
[6]  
Beyrle H., 1995, P365
[7]   Resistance of pea roots to endomycorrhizal fungus or Rhizobium correlates with enhanced levels of endogenous salicylic acid [J].
Blilou, I ;
Ocampo, JA ;
García-Garrido, JM .
JOURNAL OF EXPERIMENTAL BOTANY, 1999, 50 (340) :1663-1668
[8]   BIOCHEMICAL APPROACHES TO THE STUDY OF PLANT-FUNGAL INTERACTIONS IN ARBUSCULAR MYCORRHIZA [J].
BOTHE, H ;
KLINGNER, A ;
KALDORF, M ;
SCHMITZ, O ;
ESCH, H ;
HUNDESHAGEN, B ;
KERNEBECK, H .
EXPERIENTIA, 1994, 50 (10) :919-925
[9]   LIFE-CYCLE OF GLOMUS-INTRARADIX IN ROOT ORGAN-CULTURE [J].
CHABOT, S ;
BECARD, G ;
PICHE, Y .
MYCOLOGIA, 1992, 84 (03) :315-321
[10]   ORGAN-SPECIFIC AND ENVIRONMENTALLY REGULATED EXPRESSION OF 2 ABSCISIC ACID-INDUCED GENES OF TOMATO - NUCLEOTIDE-SEQUENCE AND ANALYSIS OF THE CORRESPONDING CDNAS [J].
COHEN, A ;
PLANT, AL ;
MOSES, MS ;
BRAY, EA .
PLANT PHYSIOLOGY, 1991, 97 (04) :1367-1374