Nanostructure analysis using spatially modulated illumination microscopy

被引：32

作者：

Baddeley, David ^{[1
]}

Batram, Claudia ^{[1
]}

Weiland, Yanina ^{[1
]}

Cremer, Christoph ^{[1
,2
,3
]}

Birk, Udo J. ^{[1
,4
,5
]}

机构：

[1] Univ Heidelberg, Kirchhoff Inst Phys, D-69120 Heidelberg, Germany

[2] Univ Heidelberg, Inst Pharm & Mol Botechnol, D-69120 Heidelberg, Germany

[3] Jackson Lab, Inst Mol Biophys, Bar Harbor, ME 04609 USA

[4] Kings Coll London, Randall Div Cell & Mol Biophys, London SE1 1UL, England

[5] FORTH, Inst Elect Struct & Laser, Iraklion 71110, Greece

来源：

NATURE PROTOCOLS | 2007年 / 2卷 / 10期

关键词：

D O I：

10.1038/nprot.2007.399

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the usage of the spatially modulated illumination (SMI) microscope to estimate the sizes (and/or positions) of fluorescently labeled cellular nanostructures, including a brief introduction to the instrument and its handling. The principle setup of the SMI microscope will be introduced to explain the measures necessary for a successful nanostructure analysis, before the steps for sample preparation, data acquisition and evaluation are given. The protocol starts with cells already attached to the cover glass. The protocol and duration outlined here are typical for fixed specimens; however, considerably faster data acquisition and in vivo measurements are possible.

引用

页码：2640 / 2646

页数：7

共 14 条

[1] Spatially modulated illumination microscopy allows axial distance resolution in the nanometer range [J].

Albrecht, B ;

Failla, AV ;

Schweitzer, A ;

Cremer, C .

APPLIED OPTICS, 2002, 41 (01) :80-87

[2] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[3] Association of pKi-67 with satellite DNA of the human genome in early G1 cells [J].

Bridger, JM ;

Kill, IR ;

Lichter, P .

CHROMOSOME RESEARCH, 1998, 6 (01) :13-24

[4] Fluorescence microscopy with super-resolved optical sections [J].

Egner, A ;

Hell, SW .

TRENDS IN CELL BIOLOGY, 2005, 15 (04) :207-215

[5] Nanosizing of fluorescent objects by spatially modulated illumination microscopy [J].

Failla, AV ;

Spoeri, U ;

Albrecht, B ;

Kroll, A ;

Cremer, C .

APPLIED OPTICS, 2002, 41 (34) :7275-7283

[6] Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution [J].

Gustafsson, MGL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (37) :13081-13086

[7] Saturated patterned excitation microscopy - a concept for optical resolution improvement [J].

Heintzmann, R ;

Jovin, TM ;

Cremer, C .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2002, 19 (08) :1599-1609

[8] BREAKING THE DIFFRACTION RESOLUTION LIMIT BY STIMULATED-EMISSION - STIMULATED-EMISSION-DEPLETION FLUORESCENCE MICROSCOPY [J].

HELL, SW ;

WICHMANN, J .

OPTICS LETTERS, 1994, 19 (11) :780-782

[9] Ultra-high resolution imaging by fluorescence photoactivation localization microscopy [J].

Hess, Samuel T. ;

Girirajan, Thanu P. K. ;

Mason, Michael D. .

BIOPHYSICAL JOURNAL, 2006, 91 (11) :4258-4272

[10]

JACKLE P, 1999, THESIS U HEIDELBERG

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