Primers with 5′ flaps improve real-time PCR

被引：67

作者：

Afonina, Irina ^{[1
]}

Ankoudinova, Irina ^{[1
]}

Mills, Alan ^{[1
]}

Lokhov, Sergey ^{[1
]}

Huynh, Phan ^{[1
]}

Mahoney, Walt ^{[1
]}

机构：

[1] Nanogen Inc, Bothell, WA 98021 USA

来源：

BIOTECHNIQUES | 2007年 / 43卷 / 06期

关键词：

D O I：

10.2144/000112631

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Primers that contain portions noncomplementary to the target region are usually used to add to the PCR product a utility sequence such as a restriction site or a universal probe binding site. We have demonstrated that primers with short 5'AT-rich overhangs increase real-time PCR fluorescent signal. The improvement is particularly significant for difficult to amplify templates, such as highly variable viral sequences or bisulfite-treated DNA.

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页码：770 / +

页数：3

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