Induction of petite mutants in yeast Saccharomyces cerevisiae by the anticancer drug dequalinium

被引：12

作者：

Schneider-Berlin, KR

Bonilla, TD

Rowe, TC ^{[1
]}

机构：

[1] Univ Florida, Coll Med, Dept Pharmacol & Therapeut, Gainesville, FL 32610 USA

[2] Univ Florida, Coll Vet Med, Dept Pathobiol, Gainesville, FL 32611 USA

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2005年 / 572卷 / 1-2期

关键词：

dequalinium; ethidium bromide; mitochondrial DNA; yeast; antifungal; anticancer;

D O I：

10.1016/j.mrfmmm.2004.12.010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Dequalinium (DEQ), a drug with both antimicrobial and anticancer activity, induced the formation of petite (respiration-deficient) mutants in the yeast Saccharomyces cerevisiae. DEQ was found to be approximately 50-fold more potent than ethidium bromide (EB) at inducing petites. Analysis of the DEQ-induced petite mutants indicated a complete loss of mitochondrial DNA (< 1 copy/cell). Prior to the loss of mtDNA, DEQ caused cleavage of the mtDNA into a population of fragments 30-40 kbp in size suggesting that this drug causes petites by inducing a breakdown of mtDNA. The selective effect of DEQ on yeast mtDNA may underlie the antifungal activity of this chemotherapeutic agent. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：84 / 97

页数：14

共 50 条

[1]

ALEXANDER NJ, 1979, J BIOL CHEM, V254, P2471

[2] SALTS OF DECAMETHYLENE-BIS-4-AMINOQUINALDINIUM (DEQUADIN) A NEW ANTIMICROBIAL AGENT [J].