Legionella pneumophila Philadelphia-1 tatB and tatC affect intracellular replication and biofilm formation

被引：63

作者：

De Buck, E ^{[1
]}

Maes, L ^{[1
]}

Meyen, E ^{[1
]}

Van Mellaert, L ^{[1
]}

Geukens, N ^{[1
]}

Anné, J ^{[1
]}

Lammertyn, E ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Rega Inst Med Res, Bacteriol Lab, B-3000 Louvain, Belgium

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 331卷 / 04期

关键词：

twin arginine; tat secretion; Legionella pneumophila; virulence; biofilms; signal peptides;

D O I：

10.1016/j.bbrc.2005.04.060

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Legionella pneumophila is a facultative intracellular human pathogen and an important cause of Legionnaires' disease, a severe form of pneumonia. Recently, we showed the presence of a putative twin-arginine translocation (Tat) pathway in L. pneumophila Philadelphia-1. This secretion pathway is used to transport completely folded proteins across the cytoplasmic membrane. The importance of the Tat pathway in L. pneumophila was investigated by constructing a tatB and a tatC mutant. Functionality of the Tat pathway was shown using a proven heterologous Tat substrate. It was shown that tatB and tatC are involved in intracellular replication in Acanthamoeba castellanii and differentiated U937 cells, and in biofilm forming ability. A Putative Legionella Tat substrate was identified via 2D gel electrophoresis. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：1413 / 1420

页数：8

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