β2-adrenergic agonist residues:: simultaneous methyl- and butylboronic derivatization for confirmatory analysis by gas chromatography mass spectrometry

被引：27

作者：

Ramos, F ^{[1
]}

Santos, C ^{[1
]}

Silva, A ^{[1
]}

da Silveira, MIN ^{[1
]}

机构：

[1] Univ Coimbra, Fac Farm, Lab Bromatol Nutr & Hidrol, P-3049 Coimbra, Portugal

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 716卷 / 1-2期

关键词：

derivatization; GC; tulobuterol; mabuterol; mapenterol; salbutamol; clenproperol; clenbuterol; clenpenterol; bromobuterol;

D O I：

10.1016/S0378-4347(98)00292-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A derivatization procedure for confirmatory residue analysis of beta(2)-agonists is described. Methyl (MBA) and butyl (BBA) boronic acids are simultaneously used for the derivatization of tulobuterol, mabuterol, mapenterol, salbutamol, clenproperol, clenbuterol, clenpenterol and bromobuterol by GC-MS determination. A temperature of 55 degrees C during 60 min was selected as optimal temperature-time condition for simultaneous MBA and BBA beta(2)-agonists derivatization. It was also observed that stability of boronic derivatives was maintained at -20 degrees C over a period of four days. The proposed methodology was tested in urine and it could be applied for confirmatory residue analysis of clenbuterol-like compounds. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：366 / 370

页数：5

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