A Rho family GTPase controls actin dynamics and tip growth via two counteracting downstream pathways in pollen tubes

被引:256
作者
Gu, Y
Fu, Y
Dowd, P
Li, SD
Vernoud, V
Gilroy, S
Yang, ZB [1 ]
机构
[1] Univ Calif Riverside, Ctr Plant Cell Biol, Inst Integrat Genome Biol, Riverside, CA 92521 USA
[2] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA
[3] Penn State Univ, Dept Biochem & Mol Biol, State Coll, PA 16802 USA
[4] Penn State Univ, Dept Biol, State Coll, PA 16802 USA
关键词
D O I
10.1083/jcb.200409140
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Tip growth in neuronal cells, plant cells, and fungal hyphae is known to require tip-localized Rho GTPase, calcium, and filamentous actin (F-actin), but how they interact with each other is unclear. The pollen tube is an exciting model to study spatiotemporal regulation of tip growth and F-actin dynamics. An Arabidopsis thaliana Rho family GTPase, ROP1, controls pollen tube growth by regulating apical F-actin dynamics. This paper shows that ROP1 activates two counteracting pathways involving the direct targets of tip-localized ROP1: RIC3 and RIC4. RIC4 promotes F-actin assembly, whereas RIC3 activates Ca2+ signaling that leads to F-actin disassembly. Overproduction or depletion of either RIC4 or RIC3 causes tip growth defects that are rescued by overproduction or depletion of RIC3 or RIC4, respectively. Thus, ROP1 controls actin dynamics and tip growth through a check and balance between the two pathways. The dual and antagonistic roles of this GTPase may provide a unifying mechanism by which Rho modulates various processes dependent on actin dynamics in eukaryotic cells.
引用
收藏
页码:127 / 138
页数:12
相关论文
共 49 条
[1]   Effectors for the Rho GTPases [J].
Aspenström, P .
CURRENT OPINION IN CELL BIOLOGY, 1999, 11 (01) :95-102
[2]   Root hair growth in Arabidopsis thaliana is directed by calcium and an endogenous polarity [J].
Bibikova, TN ;
Zhigilei, A ;
Gilroy, S .
PLANTA, 1997, 203 (04) :495-505
[3]   A CONSERVED BINDING MOTIF DEFINES NUMEROUS CANDIDATE TARGET PROTEINS FOR BOTH CDC42 AND RAC GTPASES [J].
BURBELO, PD ;
DRECHSEL, D ;
HALL, A .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (49) :29071-29074
[4]   Rho GTPases in growth cone guidance [J].
Dickson, BJ .
CURRENT OPINION IN NEUROBIOLOGY, 2001, 11 (01) :103-110
[5]   Rho GTPases in cell biology [J].
Etienne-Manneville, S ;
Hall, A .
NATURE, 2002, 420 (6916) :629-635
[6]   POLLEN SPECIFICITY ELEMENTS RESIDE IN 30 BP OF THE PROXIMAL PROMOTERS OF 2 POLLEN-EXPRESSED GENES [J].
EYAL, Y ;
CURIE, C ;
MCCORMICK, S .
PLANT CELL, 1995, 7 (03) :373-384
[7]   Reactive oxygen species produced by NADPH oxidase regulate plant cell growth [J].
Foreman, J ;
Demidchik, V ;
Bothwell, JHF ;
Mylona, P ;
Miedema, H ;
Torres, MA ;
Linstead, P ;
Costa, S ;
Brownlee, C ;
Jones, JDG ;
Davies, JM ;
Dolan, L .
NATURE, 2003, 422 (6930) :442-446
[8]   Signaling in pollination [J].
Franklin-Tong, VE .
CURRENT OPINION IN PLANT BIOLOGY, 1999, 2 (06) :490-495
[9]   Rop GTPase-dependent dynamics of tip-localized F-actin controls tip growth in pollen tubes [J].
Fu, Y ;
Wu, G ;
Yang, ZB .
JOURNAL OF CELL BIOLOGY, 2001, 152 (05) :1019-1032
[10]   Arabidopsis interdigitating cell growth requires two antagonistic pathways with opposing action on cell morphogenesis [J].
Fu, Y ;
Gu, Y ;
Zheng, ZL ;
Wasteneys, G ;
Yang, ZB .
CELL, 2005, 120 (05) :687-700