A novel PDMS microfluidic spotter for fabrication of protein chips and microarrays

被引：5

作者：

Chang-Yen, DA ^{[1
]}

Myszka, D ^{[1
]}

Gale, BK ^{[1
]}

机构：

[1] Univ Utah, Dept Mech Engn, Salt Lake City, UT 84112 USA

来源：

MICROFLUIDICS, BIOMEMS, AND MEDICAL MICROSYSTEMS III | 2005年 / 5718卷

关键词：

PDMS; spotter; microfluidic; microarray;

D O I：

10.1117/12.592151

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A novel PDMS microfluidic spotter system has been developed for the patterning of surface microarrays that require individually addressing each spot area and high probe density. Microfluidic channels are used to address each spot region and large spot arrays can be addressed in parallel. Fluorescence intensity measurement of dye-spotted samples compared to control and pipetted drops demonstrated a minimum of a three-fold increase in dye surface density. Surface plasmon resonance measurement of protein-spotted samples as compared to pin-spotted samples demonstrated an 86-fold increase in protein surface density. This novel spotter system can be applied to the production of high-throughput arrays in the fields of genomics, proteomics, immunoassays and fluorescence or luminescence assays.

引用

页码：110 / 120

页数：11

共 7 条

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