Identification of quorum-sensing-regulated genes of Burkholderia cepacia

被引：37

作者：

Aguilar, C ^{[1
]}

Friscina, A ^{[1
]}

Devescovi, G ^{[1
]}

Kojic, M ^{[1
]}

Venturi, V ^{[1
]}

机构：

[1] Int Ctr Genet Engn & Biotechnol, Bacteriol Grp, I-34012 Trieste, Italy

来源：

JOURNAL OF BACTERIOLOGY | 2003年 / 185卷 / 21期

关键词：

D O I：

10.1128/JB.185.21.6456-6462.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Quorum sensing is a regulatory mechanism (operating in response to cell density) which in gram-negative bacteria usually involves the production of N-acyl homoserine lactones (HSL). Quorum sensing in Burkholderia cepacia has been associated with the regulation of expression of extracellular proteins and siderophores and also with the regulation of swarming and biofilm formation. In the present study, several quorum-sensing-controlled gene promoters of B. cepacia ATCC 25416 were identified and characterized. A total of 28 putative gene promoters show CepR-C-8-HSL-dependent expression, suggesting that quorum sensing in B. cepacia is a global regulatory system.

引用

页码：6456 / 6462

页数：7

共 56 条

[1] Quorum-sensing system and stationary-phase sigma factor (rpoS) of the onion pathogen Burkholderia cepacia genomovar I type strain, ATCC 25416 [J].

Aguilar, C ;

Bertani, I ;

Venturi, V .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (03) :1739-1747

[2] The cell wall and cell division gene cluster in the Mra operon of Pseudomonas aeruginosa:: Cloning, production, and purification of active enzymes [J].

Azzolina, BA ;

Yuan, XL ;

Anderson, MS ;

El-Sherbeini, M .

PROTEIN EXPRESSION AND PURIFICATION, 2001, 21 (03) :393-400

[3] Quorum sensing is not required for twitching motility in Pseudomonas aeruginosa [J].

Beatson, SA ;

Whitchurch, CB ;

Semmler, ABT ;

Mattick, JS .

JOURNAL OF BACTERIOLOGY, 2002, 184 (13) :3598-3604

[4] STRUCTURAL RELATIONSHIPS AMONG RHIZOBIUM MELILOTI SYMBIOTIC PROMOTERS [J].

BETTER, M ;

LEWIS, B ;

CORBIN, D ;

DITTA, G ;

HELINSKI, DR .

CELL, 1983, 35 (02) :479-485

[5]

BURKHOLDER WH, 1950, PHYTOPATHOLOGY, V40, P115

[6] BURKHOLDERIA CEPACIA AND CYSTIC-FIBROSIS - DO NATURAL ENVIRONMENTS PRESENT A POTENTIAL HAZARD [J].

BUTLER, SL ;

DOHERTY, CJ ;

HUGHES, JE ;

NELSON, JW ;

GOVAN, JRW .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (04) :1001-1004

[7]

BUTLER SL, 1994, FEMS IMMUNOL MED MIC, V8, P285, DOI 10.1111/j.1574-695X.1994.tb00454.x

[8] Defining the Bacillus subtilis σW regulon:: A comparative analysis of promoter consensus search, run-off transcription/macroarray analysis (ROMA), and transcriptional profiling approaches [J].

Cao, M ;

Kobel, PA ;

Morshedi, MM ;

Wu, MFW ;

Paddon, C ;

Helmann, JD .

JOURNAL OF MOLECULAR BIOLOGY, 2002, 316 (03) :443-457

[9] Taxonomy and identification of the Burkholderia cepacia complex [J].

Coenye, T ;

Vandamme, P ;

Govan, JRW ;

Lipuma, JJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (10) :3427-3436

[10] Quorum-sensing signals and quorum-sensing genes in Burkholderia vietnamiensis [J].

Conway, BA ;

Greenberg, EP .

JOURNAL OF BACTERIOLOGY, 2002, 184 (04) :1187-1191

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