Postelectrophoresis capillary scanning method for DNA sequencing

被引：7

作者：

Kim, S ^{[1
]}

Yoo, HJ ^{[1
]}

Hahn, JH ^{[1
]}

机构：

[1] POHANG UNIV SCI & TECHNOL,DEPT CHEM,POHANG 790784,SOUTH KOREA

来源：

ANALYTICAL CHEMISTRY | 1996年 / 68卷 / 05期

关键词：

D O I：

10.1021/ac9508107

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A postelectrophoresis capillary scanning method for increasing the throughput of DNA sequencing has been developed, The method features a spatially and temporally separated arrangement between capillary gel electrophoresis separation of DNA sequencing fragments and the visualization of the separation pattern, Fluorescently labeled DNA sequencing fragments of pBluescript SK(-) were produced in enzymatic chain-termination reactions and separated by capillary gel electrophoresis using a 110 cm fused silica capillary with a transparent polymer coating. A partial sequence from the DNA was then determined by repeated scanning of the capillary at a rate of 8.39 cm/s, with changing excitation wavelengths and spectral filter sets.

引用

页码：936 / 939

页数：4

共 22 条

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