Multisite pooling study using ligase chain reaction in screening for genital Chlamydia trachomatis infections

被引:11
作者
Clark, AM
Steece, R
Crouse, K
Campbell, J
Zanto, S
Kartchner, D
Mottice, S
Pettit, D
机构
[1] Univ Washington, Seattle, WA 98195 USA
[2] Assoc Publ Hlth Labs, Washington, DC USA
[3] Washington State Publ Hlth Lab, Seattle, WA USA
[4] Publ Hlth Lab, Helena, MT USA
[5] Utah Div Epidemiol & Lab Serv, Salt Lake City, UT USA
[6] Div Consolidated Lab Serv, Richmond, VA USA
关键词
D O I
10.1097/00007435-200110000-00002
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Background: Ligase chain reaction (LCR), a nucleic acid amplification assay, is a highly specific and sensitive test for detecting Chlamydia trachomatis in cervical and urethral swabs as well as first-void urine specimens. Goal: To examine the suitability of using the LCR test to detect C trachomatis in pooled cervical specimens. Study Design: The performance of LCR in pooled specimens was compared with individual specimen testing at six laboratories using 3,170 cervical swab specimens randomly selected from specimens received for routine testing in the participating laboratories. These samples then were combined consecutively into 634 pools of S specimens and 317 pools of 10 specimens. A reduced sample to cutoff ratio of 0.2 or more was used for the pooled specimens. Results: Of the 188 positive specimens (98.9%), 186 were identified when single specimens were analyzed. When pools of 5 or 10 specimens were evaluated, 99.5% and 98.9% of the positive swabs, respectively, were identified correctly. Two positive specimens were detected only through pooling. Conclusions: Pooling samples for detection of C trachomatis by LCR is sensitive and specific. Depending on the prevalence of infection (positivity), LCR testing may result in cost savings, as compared with individual testing of specimens.
引用
收藏
页码:565 / 568
页数:4
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