Biomechanical effects of environmental and engineered particles on human airway smooth muscle cells

被引:54
作者
Berntsen, P. [1 ,6 ]
Park, C. Y. [1 ]
Rothen-Rutishauser, B. [3 ]
Tsuda, A. [1 ]
Sager, T. M. [1 ]
Molina, R. M. [1 ]
Donaghey, T. C. [1 ]
Alencar, A. M. [4 ]
Kasahara, D. I. [1 ]
Ericsson, T. [5 ]
Millet, E. J. [1 ]
Swenson, J. [6 ]
Tschumperlin, D. J. [1 ]
Butler, J. P. [1 ,2 ]
Brain, J. D. [1 ]
Fredberg, J. J. [1 ]
Gehr, P. [3 ]
Zhou, E. H. [1 ]
机构
[1] Harvard Univ, Sch Publ Hlth, Dept Environm Hlth, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Dept Med, Boston, MA 02115 USA
[3] Univ Bern, Inst Anat, CH-3000 Bern 9, Switzerland
[4] Univ Sao Paulo, Inst Phys, BR-05508900 Sao Paulo, Brazil
[5] Chalmers Univ Technol, Dept Math Sci, S-41296 Gothenburg, Sweden
[6] Chalmers Univ Technol, Dept Appl Phys, S-41296 Gothenburg, Sweden
关键词
nanoparticles; air pollution; environmental health; mechanobiology; cell mechanics; ULTRAFINE PARTICLES; OXIDATIVE STRESS; IN-VITRO; HUMAN LUNG; MANUFACTURED NANOPARTICLES; PARTICULATE MATTER; ENERGY-PRODUCTION; TITANIUM-DIOXIDE; SLOW DYNAMICS; SURFACE-AREA;
D O I
10.1098/rsif.2010.0068.focus
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The past decade has seen significant increases in combustion-generated ambient particles, which contain a nanosized fraction (less than 100 nm), and even greater increases have occurred in engineered nanoparticles (NPs) propelled by the booming nanotechnology industry. Although inhalation of these particulates has become a public health concern, human health effects and mechanisms of action for NPs are not well understood. Focusing on the human airway smooth muscle cell, here we show that the cellular mechanical function is altered by particulate exposure in a manner that is dependent upon particle material, size and dose. We used Alamar Blue assay to measure cell viability and optical magnetic twisting cytometry to measure cell stiffness and agonist-induced contractility. The eight particle species fell into four categories, based on their respective effect on cell viability and on mechanical function. Cell viability was impaired and cell contractility was decreased by (i) zinc oxide (40-100 nm and less than 44 mu m) and copper(II) oxide (less than 50 nm); cell contractility was decreased by (ii) fluorescent polystyrene spheres (40 nm), increased by (iii) welding fumes and unchanged by (iv) diesel exhaust particles, titanium dioxide (25 nm) and copper(II) oxide (less than 5 mu m), although in none of these cases was cell viability impaired. Treatment with hydrogen peroxide up to 500 mu M did not alter viability or cell mechanics, suggesting that the particle effects are unlikely to be mediated by particle-generated reactive oxygen species. Our results highlight the susceptibility of cellular mechanical function to particulate exposures and suggest that direct exposure of the airway smooth muscle cells to particulates may initiate or aggravate respiratory diseases.
引用
收藏
页码:S331 / S340
页数:10
相关论文
共 76 条
[1]   Do biophysical properties of the airway smooth muscle in culture predict airway hyperresponsiveness? [J].
An, Steven S. ;
Fabry, Ben ;
Trepat, Xavier ;
Wang, Ning ;
Fredberg, Jeffrey J. .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2006, 35 (01) :55-64
[2]  
[Anonymous], RES REP HLTH EFF I
[3]   Effect of welding fume solubility on lung macrophage viability and function in vitro [J].
Antonini, JM ;
Lawryk, NJ ;
Murthy, GGK ;
Brain, JD .
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A, 1999, 58 (06) :343-363
[4]   Ambient particulate pollutants in the ultrafine range promote early atherosclerosis and systemic oxidative stress [J].
Araujo, Jesus A. ;
Barajas, Berenice ;
Kleinman, Michael ;
Wang, Xuping ;
Bennett, Brian J. ;
Gong, Ke Wei ;
Navab, Mohamad ;
Harkema, Jack ;
Sioutas, Constantinos ;
Lusis, Aldons J. ;
Nel, Andre E. .
CIRCULATION RESEARCH, 2008, 102 (05) :589-596
[5]   ABNORMALITIES IN AIRWAY SMOOTH-MUSCLE IN FATAL ASTHMA [J].
BAI, TR ;
PRASAD, FW .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1990, 141 (03) :552-557
[6]   Toxicological hazards of inhaled nanoparticles - Potential implications for drug delivery [J].
Borm, PJA ;
Kreyling, W .
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2004, 4 (05) :521-531
[7]   Cytoskeletal remodelling and slow dynamics in the living cell [J].
Bursac, P ;
Lenormand, G ;
Fabry, B ;
Oliver, M ;
Weitz, DA ;
Viasnoff, V ;
Butler, JP ;
Fredberg, JJ .
NATURE MATERIALS, 2005, 4 (07) :557-561
[8]   Cytoskeleton dynamics: Fluctuations within the network [J].
Bursac, Predrag ;
Fabry, Ben ;
Trepat, Xavier ;
Lenormand, Guillaume ;
Butler, James P. ;
Wang, Ning ;
Fredberg, Jeffrey J. ;
An, Steven S. .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 355 (02) :324-330
[9]   Chronic exposure to high levels of particulate air pollution and small airway remodeling [J].
Churg, A ;
Brauer, M ;
Avila-Casado, MD ;
Fortoul, TI ;
Wright, JL .
ENVIRONMENTAL HEALTH PERSPECTIVES, 2003, 111 (05) :714-718
[10]   Fast and slow dynamics of the cytoskeleton [J].
Deng, Linhong ;
Trepat, Xavier ;
Butler, James P. ;
Millet, Emil ;
Morgan, Kathleen G. ;
Weitz, David A. ;
Fredberg, Jeffrey J. .
NATURE MATERIALS, 2006, 5 (08) :636-640