Identification and phylogenetic analysis of orf virus from goats in Taiwan

被引:75
作者
Chan, Kun-Wei
Lin, Jen-Wei
Lee, Shu-Hwae
Liao, Chih-Jung
Tsai, Meng-Chun
Hsu, Wei-Li
Wong, Min-Liang [1 ]
Shih, Hung-Chang
机构
[1] Natl Chung Hsing Univ, Coll Vet Med, Dept Vet Med, Taichung 402, Taiwan
[2] Council Agr, Anim Hlth Res Inst, Tamsui 251, Taiwan
[3] Natou Anim Dis Control Ctr, Natou 540, Taiwan
[4] Natl Chung Hsing Univ, Coll Vet Med, Grad Inst Vet Publ Hlth, Taichung 402, Taiwan
[5] China Med Univ Hosp, Dept Lab Med, Taichung 404, Taiwan
关键词
orf virus; B2L gene; phylogenetic analysis;
D O I
10.1007/s11262-007-0144-6
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
An outbreak of contagious ecthyma in goats in central Taiwan was investigated. The disease was diagnosed by physical and histopathologic examinations, and the etiology of the disease was identified as orf virus by electron microscopy and polymerase chain reaction (PCR) and sequence of major envelope protein (B2L) gene. The entire protein-coding region of B2L gene were cloned and sequenced. Phylogenetic analysis of B2L amino acid sequences showed that the orf virus identified in this outbreak was closer to the Indian ORFV-Mukteswar 59/05 isolate. This is the first report on the molecular characterization of orf virus in Taiwan.
引用
收藏
页码:705 / 712
页数:8
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