Variability of Botrytis cinerea sensitivity to pyrrolnitrin, an antibiotic produced by biological control agents

被引:16
作者
Ajouz, S. [1 ]
Walker, A. S. [2 ]
Fabre, F. [1 ]
Leroux, P. [2 ]
Nicot, P. C. [1 ]
Bardin, M. [1 ]
机构
[1] INRA, Unite Pathol Vegetale, UR407, F-84140 Montfavet, France
[2] INRA, Bioger CPP, UMR 1290, F-78850 Thiverval Grignon, France
关键词
Biological control; Durability; Baseline; Multiple-drug-resistance; Aggressiveness; ABC TRANSPORTER BCATRB; BROAD-SPECTRUM; GRAY MOLD; FUNGICIDE SENSITIVITY; POSTHARVEST CONTROL; POWDERY MILDEW; GREY MOLD; BLUE MOLD; RESISTANCE; DEFENSE;
D O I
10.1007/s10526-010-9333-7
中图分类号
Q96 [昆虫学];
学科分类号
摘要
To establish a baseline sensitivity of Botrytis cinerea to pyrrolnitrin, an antibiotic produced by several biological control agents, 204 isolates were tested for sensitivity to pyrrolnitrin using a spore germination assay. The results showed that the isolates exhibited a wide range of sensitivity to pyrrolnitrin, with an 8.4-fold difference in EC50 (effective concentration to reduce spore germination by 50% comparing to the control) values between the least and the most sensitive isolates. The model-based clustering analysis indicates that the distribution of the EC50 values best fit a normal mixture model with three components and unequal variance. The less sensitive isolates were also multidrug resistant isolates. The efficacy of the pyrrolnitrin-producing Pseudomonas chlororaphis ChPhzS24 strain was tested in vitro and on tomato plants with isolates of B. cinerea having different EC50 values. Whatever the EC50 value of the isolates tested, no significant differences in sensitivity were observed towards this bacterium indicating an absence of resistance to this biological control agent within B. cinerea isolates and suggesting also that additional mechanisms of action are probably operated by this biological control agent.
引用
收藏
页码:353 / 363
页数:11
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