Biotin-streptavidin-induced aggregation of gold nanorods: Tuning rod-rod orientation

被引:132
作者
Gole, A [1 ]
Murphy, CJ [1 ]
机构
[1] Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA
关键词
D O I
10.1021/la0512704
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We report herein biotin-streptavidin-mediated aggregation studies of long gold nanorods. We have previously demonstrated end-to-end linkages of gold nanorods driven by the biotin-streptavidin interaction (Caswell et al. J. Am. Chem. Soc. 2003, 125, 13914). In that report, the specific binding of biotin disulfide to the gold nanorod edges was achieved due to the preferred binding of thiol molecules to the Au{111} surface (gold nanorod ends) as opposed to the gold nanorod side faces. This led to the end-end linkage of gold nanorods upon subsequent addition of streptavidin. In this report we demonstrate a simple procedure to biotinylate the entire gold nanorod surface and subsequently form a 3-D assembly by addition of streptavidin. Gold nanorods were synthesized by the three-step seeding protocol documented in our previous articles. The surface of gold nanorods was further modified by a layer of a weak polyelectrolyte, poly(acrylic acid), PAA. A biotin molecule which has an amine group at one end (biotin-PEO-amine) was anchored to the carboxylic acid group of the polyelectrolyte using the well-known carbodiimide chemistry. This process biotinylates the entire gold nanorod surface. Addition of streptavidin further leads to aggregation of gold nanorods. A closer look at the aggregates reveals a preferential side-to-side assembly of gold nanorods. The gold nanorods were characterized at each stage by UV-vis spectroscopy, light scattering, and transmission electron microscopy (TEM) measurements.
引用
收藏
页码:10756 / 10762
页数:7
相关论文
共 60 条
[1]   Organization of 'nanocrystal molecules' using DNA [J].
Alivisatos, AP ;
Johnsson, KP ;
Peng, XG ;
Wilson, TE ;
Loweth, CJ ;
Bruchez, MP ;
Schultz, PG .
NATURE, 1996, 382 (6592) :609-611
[2]   ADSORPTION OF WEAK POLYELECTROLYTES ON HIGHLY CHARGED SURFACES - POLY(ACRYLIC ACID) ON POLYSTYRENE LATEX WITH STRONG CATIONIC GROUPS [J].
BLAAKMEER, J ;
BOHMER, MR ;
STUART, MAC ;
FLEER, GJ .
MACROMOLECULES, 1990, 23 (08) :2301-2309
[3]   SYNTHESIS OF THIOL-DERIVATIZED GOLD NANOPARTICLES IN A 2-PHASE LIQUID-LIQUID SYSTEM [J].
BRUST, M ;
WALKER, M ;
BETHELL, D ;
SCHIFFRIN, DJ ;
WHYMAN, R .
JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1994, (07) :801-802
[4]   Preferential end-to-end assembly of gold nanorods by biotin-streptavidin connectors [J].
Caswell, KK ;
Wilson, JN ;
Bunz, UHF ;
Murphy, CJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (46) :13914-13915
[5]  
CHANG JY, 2005, J CHEM SOC CHEM COMM, V8, P1092
[6]   Large-scale growth and end-to-end assembly of silver nanorods by PVP-directed polyol process [J].
Chen, DL ;
Gao, L .
JOURNAL OF CRYSTAL GROWTH, 2004, 264 (1-3) :216-222
[7]   SEMICONDUCTOR NANOCRYSTALS COVALENTLY BOUND TO METAL-SURFACES WITH SELF-ASSEMBLED MONOLAYERS [J].
COLVIN, VL ;
GOLDSTEIN, AN ;
ALIVISATOS, AP .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (13) :5221-5230
[8]   Vibrational coherence of self-organized silver nanocrystals in f.c.c. supra-crystals [J].
Courty, A ;
Mermet, A ;
Albouy, PA ;
Duval, E ;
Pileni, MP .
NATURE MATERIALS, 2005, 4 (05) :395-398
[9]   Gold nanoparticles: Assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology [J].
Daniel, MC ;
Astruc, D .
CHEMICAL REVIEWS, 2004, 104 (01) :293-346
[10]   Fuzzy nanoassemblies: Toward layered polymeric multicomposites [J].
Decher, G .
SCIENCE, 1997, 277 (5330) :1232-1237