A PCR-based assay to detect hAT-like transposon sequences in plants

被引：7

作者：

De Keukeleire, P

De Schepper, S

Gielis, J

Gerats, T

机构：

[1] State Univ Ghent, Fac Sci, Dept Plant Syst Biol, B-9000 Ghent, Belgium

[2] Geniaal Bvba, B-2018 Antwerp, Belgium

[3] State Univ Ghent, Fac Appl Biol & Agr Sci, Dept Plant Prod, B-9000 Ghent, Belgium

来源：

CHROMOSOME RESEARCH | 2004年 / 12卷 / 02期

关键词：

hAT-like transposon; phylogeny; plant genomics; polymerase chain reaction (PCR);

D O I：

10.1023/B:CHRO.0000013163.34505.96

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Despite their potential as endogenous tools for forward and reverse genetics, members of the hobo, Ac, Tam3 (or hAT) superfamily of transposable elements have been characterized in but a limited number of plant species. To expedite their isolation, we developed a PCR-based assay for the detection of hAT-like transposon sequences in plants which was applied to isolate and initially characterize such sequences from Petunia hybrida, Phaseolus vulgaris, Bambusa vulgaris, Brassica napus and Rhododendron simsii.

引用

页码：117 / 123

页数：7

共 31 条

[21] ACTIVITY OF THE TRANSPOSON TAM3 IN ANTIRRHINUM AND TOBACCO - POSSIBLE ROLE OF DNA METHYLATION [J].