Enzyme sensors prepared by layer-by-layer deposition of enzymes on a platinum electrode through avidin-biotin interaction

被引：52

作者：

Anzai, J ^{[1
]}

Kobayashi, Y ^{[1
]}

Suzuki, Y ^{[1
]}

Takeshita, H ^{[1
]}

Chen, Q ^{[1
]}

Osa, T ^{[1
]}

Hoshi, T ^{[1
]}

Du, XY ^{[1
]}

机构：

[1] Tohoku Univ, Fac Pharmaceut Sci, Aoba Ku, Sendai, Miyagi 980857, Japan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1998年 / 52卷 / 1-2期

关键词：

enzyme multilayer; avidin-biotin system; biotin-labeled enzyme; enzyme sensor; glucose sensor; lactate sensor;

D O I：

10.1016/S0925-4005(98)00248-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An alternate and repeated deposition of avidin and biotin-labeled glucose oxidase (GOx) or lactate oxidase (LOx) on a solid surface gave protein multilayers through a strong binding between avidin and biotin. Spectrophotometric and gravimetric techniques using UV absorption and a quartz-crystal microbalance revealed that the protein multilayers are composed of avidin monolayers and enzyme monolayers which in turn are built in a layer-by-layer structure. The GOx and LOx enzymes were found to be catalytically active in the multilayer films, by means of a cyclic voltammetry. The response characteristics of the GOx- and LOx-modified sensors are discussed. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：3 / 9

页数：7

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